HORT 301 L4

Gram-negative bacteria

thin, peptoglycan layer, cannot see gram-negative or not in KOH test

Gram-positive bacteria

thick peptoglyacan layer, no outer membrane

Quorum Sensing in bacteria

bacteria communicate cell-to-cell after penetrating and replicating in the host

Ooze

a mixture of broken-down host cell contents, sap, and dense bacterial masses

Prokaryote

simple, single-celled organism that does not have a distinct nucleus or other membrane-bound internal parts

What do bacteria cells not have?

nucleus, mitochondria, chloroplasts, or other membrane-bound organelles

DNA in bacteria

t

Typical form of reproduction for bacteria

budding and fission

How long does it take bacterial population to double in a plant?

What do bacteria cells have that Eukaryotic cells do not?

How do bacteria get their nutrients?

breaking down cells into wet jelly they like, then absorbing its nutrients

Virulent

how active the pathogen cells are

Methods bacteria is spread

plant debris, soil, cankers, tools, seeds, insects, water

What do bacteria produce to protect them from exposure to UV or other harmful agents that allow them to survive on their own for a short amount of time?

peptides

What kinds of wounds do bacteria get into?

stomates or hydathodes

What are hydathodes?

openings along the margin of plants where excess water is expelled

What do bacteria look for once inside the host?

specific nutrient conditions

What do bacteria evade once in the cell?

recognition by the plant

What do bacteria do after evading recognition?

signs and symptoms of bacteria

ooze, leaf spots, leaf blights, leaf scorch, wilted plants, galls, cankers, vascular discoloration

two most popular disease examples

bacteria tests

ooze test

lab culturing (quadrant test)

KOH test

potato rot test

lab test

Elisa

staining test

positive (gram-negative) ooze test

negative (gram-positive)

KOH test

wait for mucous membrane in KOH solution

positive KOH is for positive (gram negative bacteria)

potato rot test

rub bacteria on potato and see if it rots

Ralstonia solanacearum

bacterial wilt of tomato and has brown discoloration of the vasular tissue

also infects through water: tomato, tobacco, potato, eggplant, pepper, sunflower

management of bacteria spot and wilt

bacterial wilt from Erwinia tracheiphila

One insect vector that can move or spread bacteria?

cucumber beetle

Management strategies of bacteria wilt