Lab 8

what does anaerobic mean?

an absence of oxygen; not oxygen dependent for growth

What are we going to use fluid thioglycollate medium to indicate?

to determine the oxygen requirements of a given species of bacteria; eliminates oxygen from a culture

How will we make jar anaerobic?

hydrogen generator packet added to jar along with catalyst. hydrogen reacts with the oxygen in jar, forming water which appears as condensation along the sides of the jar. methylene blue strip or pellet used to see if oxygen is removed from the jar

facultative anaerobe

facultative aerobe

aerotolerant anaerobe

microaerophile

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what does the oxidase test tell us about bacteria?

described what a positive and negative results looks like

catalase breaks down __, producing __ and __

how can one tell if catalase is acting on it substrate?

What does a color change in a phenol red broth indicate about a bacterium that has been growing in it?

phenol red: neutral pH color

phenol red: acidic pH color

phenol red: alkaline pH color

what is a Durham tube?

what does it collect?

what two products may be produced during the fermentation of simple carbohydrates?

how is each identified in the fermentation test? Can you have one without the other?

Name 3 simple carbohydrates that can be added to media for biochemical testing