Animal Cell, Tissue and Organ Culture

These flashcards cover key concepts related to animal cell culture, methods, and applications.

Animal cell culture is a type of biotechnological technique where animal cells are artificially grown in a __.

favorable environment.

The cells used in animal cell culture are usually obtained from __ eukaryotes and their established cell lines.

multicellular.

The cells in primary cell culture are __-growing cells that carry all the characteristics of the original tissue or cells.

slow.

Primary cell cultures are prone to __ by bacteria and viruses.

contamination.

Secondary cell cultures are obtained after the primary cell cultures are subsequently __ over a period of time in fresh culture media.

subcultured.

Continuous cell lines are cells that exhibit __ growth via subsequent subcultures.

indefinite.

Finite cell lines can divide from to times before they eventually die.

20; 100

The __ Phenomenon refers to the finite number of times normal human somatic cells can divide in cell culture before entering a state of senescence.

Hayflick.

Animal cell cultures can be used for the production of __ and antibodies.

large quantities of proteins.

The development of animal cell culture is critical for the advances in __ therapy.

gene.

The ability of the cells to grow as suspension depends on the __ of cells as cells that remain as suspensions in the body are effective suspension cells.

source.

Cell Lines can be further divided into two groups based on the __ patterns of the cells.

growth.