DEHYDRATION (LAB)

DEHYDRATION

the removal of intercellular and intracellular water content from fixated / decalcified tissues, performed prior to impregnation.

DEHYDRATION

agents usually in the form of alcohol with varying concentrations

DEHYDRATION

Starts from 70% Ethanol in water, progressing through 95% Ethanol to 100% Ethanol

DEHYDRATION

Keep the time as brief as possible to avoid the extraction of cellular constituents

DEHYDRATION

The amount of the agent in each stage should not be less than 10 times the volume of the tissues in order to ensure complete penetration

Embryonic tissues

dehydration starts with 30% Ethanol

ETHANOL

For routine dehydration of tissues

ETHANOL

best dehydrating agent

ETHANOL

Non-toxic and not very expensive

METHANOL

Toxic, for blood and tissue films

BUTYL ALCOHOL

Used in plant and animal micro-techniques, recommended for tissues that do not require rapid processing

ACETONE

Cheap, rapid dehydrating agent used for most urgent biopsies

ACETONE

Most lipids are removed from the tissues

ACETONE

extremely volatile and flammable.

ACETONE

Not recommended as a routine dehydrating agent

DIOXANE (DIETHYLENE DIOXDE)

Excellent dehydrating and clearing agent readily miscible in water, melted paraffin, alcohol and xylol

DIOXANE (DIETHYLENE DIOXDE)

Tissues may be placed directly into ______ right after washing out, but may tend to ribbon poorly.

DIOXANE (DIETHYLENE DIOXDE)

Extremely dangerous (Vapors produce a cumulative and highly toxic action in man)

CELLOSOLVE (ETHYLENE GYCOL MONOETHYL ETHER)

Reproductive, Fetal, Urinary and Blood systems are particularly vulnerable to the toxic side effects

CELLOSOLVE (ETHYLENE GYCOL MONOETHYL ETHER)

Propylene – based glycol ethers should be used instead of ethylene based glycols.

TETRAHYDROFURAN

Dissolves fat, also miscible in lower alcohols, ether, chloroform, acetone, benzene and xylene

TETRAHYDROFURAN

a reagent that both dehydrates and clears tissues; miscible in both water and paraffin

TETRAHYDROFURAN

Toxic when inhaled or ingested. Vapors cause dizziness, headache and anesthesia.

TETRAHYDROFURAN

It is an eye irritant, prolonged exposure like in 6 months may cause conjunctival irritation.

4% Phenol to 95% Ethanol baths

gives a softening effect to hard tissues like tendons, nails or dense fibrous tissues.

Glycerol/Alcohol mixture or in Molliflex

Hard tissues can be placed in a