Plasma Proteins

Plasma protein associated with transports T3 and T4; negative APR

Prealbumin

Other name of prealbumin

Transthyretin

Markers for poor nutritional status

Prealbumin, Albumin

Sensitive and highly prognostic marker for Cystic Fibrosis

Albumin

Term referred to the absence of albumin (congenital)

Analbuminemia

TP-Albumin = ?

Globulin

This is 90% of alpha globulin component

a1-Antitrypsin

Plasma protein associated with natural defense; inhibits protease trypsin (neutrophil elastase); helps fight infection

a1-Antitrypsin

Plasma protein associated with low levels seen in Trisomy 18, 21

a1-fetoprotein

AFP is detectable in maternal serum up to how many months of pregnancy

7th-8th month of pregnancy

Increased levels of this indicate hepatocellular tumor and gonadal tumors in adults

a1-fetoprotein

Plasma protein associated with negatively charged even in ACIDIC environment

a1-acid glycoprotein

Plasma protein associated with AKA OROSOMUCOID

a1-acid glycoprotein

Plasma protein associated with useful diagnostic tool in neonates with bacterial infection

a1-acid glycoprotein

Plasma protein associated with affinity for progesterone, binds quinidine (cardioactive drug)

a1-acid glycoprotein

Plasma protein associated with binds with PSA; increased in alzheimers; parkinson’s disease

a1-antichymotrypsin

Plasma protein associated with serine proteinase inhibitor family

a1-antichymotrypsin, inter-a1-trypsin inhibitor

Plasma protein associated with major carrier protein of VIT D; transports FAs and endotoxins; increased in 3rd trimester pregnancy

GC-globulin

Plasma protein associated with wilson’s disease

Ceruloplasmin

Relationship of ceruloplasmin and copper

Indirect relationship

Plasma protein associated with Menke’s syndrome; kinky hair disease

Ceruloplasmin

Major component of beta-globulin

Transferrin

Plasma protein associated with transports iron to storage sites and to cells; increased in iron deficiency anemia

Transferrin

Autosomal recessive disorder with absence of transferrin

Atransferrinemia

Transferrin is increased or decreased in inflammation?

Decreased

Plasma protein associated with removes circulating heme, ferriheme, porphyrin

Hemopexin

Plasma protein associated with diagnosis of early hemolysis

Hemopexin

Plasma protein associated with protects against OXIDATIVE DAMAGE

Hemopexin

LDL appears as a separate band in the _ region

Beta-globulin region

Plasma protein associated with light chain component of the major histocompatibility complex; found in surface of MOST nucleated cells; high in lymphocytes

B2-microglobulin

Plasma protein associated with SERO TERMS

B2-microglobulin

Complement is increased or decreased in DIC, malnutrition, hemolytic anemia

Decreased

Most abundant and 2nd most abundant complement protein in human serum

C3: most abundant

C4: 2nd

Plasma protein associated with responsible for fibrin clot; ONLY PROTEIN FOUND IN PLASMA AND NOT IN SERUM

Fibrinogen

Immunoglobulins are composed of how many heavy and light chains

2 heavy; 2 light

Identify the Immunoglobulin: first to appear, pentamer

IgM

Identify the Immunoglobulin: can cross placenta

IgG

Identify the Immunoglobulin: mucus secretion

IgA

Identify the Immunoglobulin: allergies and anaphylactic reactions

IgE

Plasma protein associated with O2 carrying protein found in striated skeletal muscle

Myoglobin

Plasma protein associated with found in highest conc. in left ventricular myocardium; also found in atrial tissue & right ventricle

Brain natriuretic peptide

Protein measurement method associated with acid digestion of protein with measurement of total NITROGEN

Kjeldahl

NH3 measurement may use what 2 principles/methods in Kjeldahl

Nessler’s, Berthelot’s

Kjeldahl is heated to what temp

340-365C

Protein measurement method associated with most widely used method

Biuret

Bond associated with Biuret method

Peptide bonds

Biurate reagents that:

• breaks the peptide bonds

• keeps copper in solution

• stabilizes cupric ion (antioxidant)

Cupric ions - break peptide bonds

Tartrate salts - keeps copper in solution

Potassium iodide - stabilizes cupric ion

Refractive index of H2O

1.330

Protein measurement method associated with bromphenol blue, ponceau S, amido black 10B, lissamine green, coomasie brilliant blue 250

Dye binding

CHONs absorb UV at what nm

200-280nm

Most CHONs are absorbed at 200nm, but has to be extended to 280nm, because?

Because of aromatic rings (TRP, TYR, PHE)

Fractionation by electrophoresis uses _ with pH of 8.6

Barbital (veronal)

Protein band pattern associated with rock n roll

Monoclonal gammopathy

Protein band pattern associated with increased a2, beta fractions; low albumin

Nephrotic syndrome

Protein band pattern associated with beta-gamma bridge

cIRRHOSIS

Abnormal protein seen in patients with MULTIPLE MYELOMA

Bence Jones protein