Exp. 10: Carbohydrates Hydrolysis

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24 Terms

1

Starch

What is the most important source of carbohydrates in the human diet, accounting for more than 50% of carbohydrate intake?

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2

Amylose

What polymer in starch is a linear polysaccharide composed entirely of D-glucose units joined by α-1,4-glycosidic linkages?

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3

Amylopectin

What polymer in starch is a branched polysaccharide with glucose units linked primarily by α-1,4-glycosidic bonds and occasional α-1,6-glycosidic bonds?

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4

Blue-black

What color appears when iodine reacts with amylose due to its coiled structure?

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5

Reddish-brown

What color is observed when iodine reacts with amylopectin?

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6

Amylase

What enzyme hydrolyzes starch into shorter polysaccharides, dextrins, maltose, and glucose?

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7

Hydrolysis

What is the chemical reaction called where carbohydrates are broken down into glucose by reacting with water?

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8

Glycogen

What is the energy reserve carbohydrate of animals, abundant in the liver and skeletal muscle cells?

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9

Both are branched-chain polysaccharides, but glycogen is more highly branched.

What is the structural similarity between glycogen and amylopectin?

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10

Reddish-brown

What color does glycogen produce when treated with iodine?

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11

Glycolysis

What is the process in which glucose is broken down to produce energy?

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12

Iodine Test

What test distinguishes starch from monosaccharides, disaccharides, and other polysaccharides using iodine?

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13

Bluish-black color

In the iodine test, what indicates a positive result for starch?

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14

Yellowish-brown color (no change)

What indicates a negative result for starch in the iodine test?

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15

Amylase cannot attack the branching (1→6) linkages in glycogen.

During the hydrolysis of carbohydrates, why can't amylase fully digest glycogen?

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16

(1) Formation of branched oligosaccharides

(2) Formation of dextrin, maltose, and glucose.

What are the two stages in the degradation of starch?

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17

Deep blue-black color

What is the expected theoretical result of starch treated with Lugol's iodine at 0 minutes?

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18

Reddish-brown color

What is the expected theoretical result of glycogen treated with Lugol's iodine at 0 minutes?

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19

Blue color

What is observed at 6 minutes when starch is treated with Lugol's iodine during hydrolysis?

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20

Yellowish-brown color

What is observed at 6 minutes when glycogen is treated with Lugol's iodine during hydrolysis?

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21

Very pale blue or colorless

What is the final color change observed for starch after 12 minutes in the iodine test during hydrolysis?

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22

Colorless

What is the final color change observed for glycogen after 12 minutes in the iodine test during hydrolysis?

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23

Due to the strong complex formed between iodine and the coiled structure of amylose.

Why does starch show a deep blue-black color with iodine initially?

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24

The branching in glycogen reduces iodine binding efficiency.

Why does glycogen produce a weaker color reaction with iodine compared to starch?

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