110.4 test

1. What are the three main uses or tests that are performed on a peripheral blood smear in Hematology?

Differential count of WBCs and WBC count estimate
Study RBC morphology
Platelet morphology and platelet count estimate

3. What are the five characteristics of a well-made blood smear?

No pool at origin
Gradual transition from thick to thin
Parallel sides
Margins on both sides of the slide
Feathered edge
Length: 1 ½ -2 inches
No holes/waves/streaks

A. Taking the spreader back too fast:

decrease spreader angle

B . Taking the spreader slide back too slow:

increase speed and use even, smooth movement

C. Holding the spreader slide higher than a 30 degree angle:

decrease spreader angle

D. Holding the spreader slide lower than a 30 degree angle:

increase angle of spreader slide

5. What is the stain which is used in Hematology to stain blood films?

Wrights stain

6. What is the pH range of the buffer used in the usual stain for Hematology?

6.4-6.8

7. Wright's-Giemsa is used to stain what?

to stain parasites

8. What types of granules stain orange-red (specific or non-specific)?

SEGS

9. What types of granules stain orange-red granules?

RBC

10. What cell is best identified because it has blue-black or deep purple granules?

BASOs

11. What could be done with a stain that is too acidic?

Increase staining time and decrease amount of buffer which is adder to slide; decrease wash time

12. What correction should you make in your technique of preparing blood films/ smears if your blood extends to the end of the end of the slide and a large blob of blood remains where your feathered edge should be?

Use a smaller drop

13. What is the basic component of Wright's stain and what specific granule loves the dye?
A. component name-

modified Rownanowsky stain

15. Describe the appearance of the area on the blood smear that should be used to count differentials.

The thin, feathered edge of the blood smear, under the microscope the red cells completely cover the field but do not or only slightly overlap

16. What is the time limit that blood films can be made from a collected EDTA tube?

>3-4 hours old at room temperature

17. If blood smears are made incorrectly what cells will be seen on the edges of the smear and what cells will be increased in the middle portion of the smear?

Increase segs, monos, and large abnormal cells at the edges and tail of smear.

18. What happens to platelets if the smear is made incorrectly?

platelets clumped at the edges of the smear making the platelets appear decrease in the normal counting area

20. What are the four series of leukocytes that are normally found in the peripheral blood?

A. myelocytic (granulocytic): neutrophils, eosinophils, basophils
B. monocytic
C. lymphocytic
D. plasmacytic

21. What series of leukocyte is not normally found in the peripheral blood but is found in the blood marrow?

plasma cells

22. What three cells are considered to be granulocytes because they contain specific granules and are in the granulocytic or myelocytic series?

Neutrophil, eosinophil, basophil- myelocytic series