Immuno Lab 9: RH factor

what serological technique are we using

latex agglutination

rheumatoid arthritis

Autoimmune disease in which the immune system attacks the synovium that lines the joints

who are more likely to have RA

women

age of onset

20-40

age of prevalence

65

when is the pain most severe

morning (stiffness)

rheumatoid factor (RF) is found in

80% of RA patients

RF is _____ with RA but it is not ______ of RA

associated; cause

RF is what isotype

Anti-IgG

what is RF

Anti-IgG (anti- antibody)

functions as an antigen while being an antibody

what are the different isotypes of RF

• IgM

• IgA

• IgG

what isotype is detectable using latex

IgM

what titer suggest RA

> 80

what is the known

antigen- IgG (reagent)

what is the unknown

antibody- RF (patient sample)

false positive can be caused by

Leprosy SHITS

• leprosy

• syphilis

• hepatitis

• infectious mononucleosis

• tuberculosis

• SLE

what can cause a false negative

• patient maybe have IgG or IgA form of RF

• a high titer (prozone)

• 20% of patient with RA do not have RF

what is the specificity

> 95%

can pain/inflammation occur symmetrically

yes but it won’t always