Micro exam biochemical reactions

Staphylococcus aureus

BA: medium, round, golden-yellow colonies, beta

MSA: yellow colonies

Catalase: +

coagulase: +

DNAse: +

Oxidase: =

Group B streptococcus

BA: small, grey-white colonies, narrow zone of beta-hemolysis

catalase: =

CAMP: +

Hippurate hydrolysis: +

bacitracin sensitivity: =

bile esculin: =

PYR: =

Group A strep

BA: Small, white-gray colonies, wide zone of beta hemolysis

Catalase: =

bacitracin: +

PYR: +

CAMP: =

Hippurate hydrolysis: =

esculin hydrolysis: =

Enterococcus faecalis

BA: small, greyish colonies

catalase: =

bile esculin agar: +

salt tolerance (6.5% NaCl): +

PYR: +

Motility: =

Haemophilus influenzae

choc: smooth, moist, grayish colonies on chocolate agar

X and V: +

catalse: +

oxidase: +

indole: +

ALA: +

latex agglutination: possible type B capsule

Escherichia coli

MAC: pink colonies

BA: gray, moist colonies, no hemolysis

oxidase: =

catalase: +

lactose ferm: +

indole: +

citrate: =

urease: =

H2S: =

MR: +

VP: =

Motility: +

Pseudomonas aeruginosa

BA: large, flat colonies with metallic sheen and beta hem

MAC: non-lactose fermenting, colorless colonies

pyocyanin, grape-like smell

oxidase: +

catalase: +

glucose ferm: =

nitrate reduc: +

motility: +

growth at 42 C: +

citrate: +

Klebsiella pneumoniae

BA: large, mucoid, gray-white colonies

MAC: large, pink, mucoid colonies

Oxidase: =

catalase: +

indole: =

citrate: +

urease: +

motility: =

VP: +

MR: =

Yersinia enterocolitica

CIN: colonies with bull’s eye appearance

MAC: colorless

oxidase: =

catalase: +

urease: +

motility as 25 C: +

TSI: A/A - orange on top

lysine decarboxylase: =

sorbitol ferm: =

ONPG: +

Vibrio parahaemolyticus

TCBS: large, green colonies

BA: smooth, translucent colonies

halophilic

oxidase: +

motility: +

growth in 0% NaCl: =

growth in 6% NaCl: +

sucrose: =

string test: +

Lysine decarboxylase: +

ornithine decarboxylase: +

Burkholderia cepacia

BA: smooth, glossy, slightly wrinkled colonies with yellow-green pigmentation

MAC: clear colonies

oxidase: weakly positive

catalase: +

motility: +

oxidative metabolism: +

ADH: =

DNAse: =

Growth at 42C: +

Moraxella catarrhalis

BA: gray-white, smooth convex colonies, no hem

Choc: enhanced growth

oxidase: +

catalase: +

DNAse: +

Butyrate esterase: +

carboydrate utilization: =

MAC growth: =

Stenotrophomonas maltophilia

BA: lavender-green colonies

MAC: Non-lactose fermenting pale colonies

oxidase: =

catalse: +

motility: +

DNAse: +

Oxidative metabolism: =

TMP-SMX: sensitive

Acinetobacter baumannii

BA: smooth opaque, convex, non-hemolytic colonies

MAC: bluish, cornflower, non-lactose fermenting colonies

Oxidase: =

Catalase: +

motility: =

glucose oxidation: +

Proteus mirabilis

BA: large, gray colonies with swarming motility, chocolate-like odor

MAC: colorless colonies

oxidase: =

catalase: +

urease: +

indole: =

H2S: +

Phenylalanine deaminase: +

Candida albicans

SDA: creamy, shite, smooth colonies

CHromogenic agar: green colonies

germ tube: +

chlamydospore formation on cornmeal agar: +

urease =

Staphylococcus saprophyticus

BA: white to yellowish, non-hemolytic colonies

catalase: +

coagulase: =

novobiocin susceptibility: =

urease: +

serratia marcescens

BA: smooth colonies with red pigment

MAC: non-fermenter

Oxidase: =

catalase: +

motility: = 

DNAse: +

Streptococcus pneumoniae

BA: small, round, alpha hem, umbonate colonies

catalase: =

optochin sensitivity: +

bile solubility: +

Capsule: +

Staphylococcus epidermidis

BA: small, white, non-hemolytic colonies

catalase: +

coagulase: =

Novobiocin susceptibility: +

mannitol fermentation: =

biofilm: +

Candida glabrata

SDA: white to cream-colored, smooth colonies

chromogenic: pink to purple colonies

germ tube: =

pseudohyphae: =

urease: =

glucose and triatose assimilation: +

chlamydospore: =

Coccidioides immitis

arthroconidic

valley fever

Pasteurella multocida

BA: large, gray-white colonies

choc: enhanced

oxidase: +

catalase: +

indole: +