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Demonstrated that air was not the cause of spoilage
Louis Pasteur
Developed the first microscope
Anton Van Leeuwenhoek
Father of canning
Nicholas Appert
Received the Nobel Prize in 1905 for demonstrating that a particular organism is responsible for a disease and that it can be transmitted from one animal to another
Robert Koch
A Pathogen does what?
Makes you sick
Bacterial ________________ is responsible for the slimy "texture" of spoiled fish or chicken.
Capsule
Viruses replicate in food and living cells.
False
Microorganisms are capable of producing which of the following.
Acids, CO2, Toxins, Alcohol
An acid produced during spoilage of milk causes
Protein Denaturation
Assume that a given food had a population of 150,000 microorganisms per ml. How many colonies would form on plate #4 above?
150
Assume that a given food had a population of 150,000 microorganisms per ml. How many colonies would form on plate #5 above?
15
Assume that a given food had a population of 150,000 microorganisms per ml. Which plate should be counted to determine the # in the original sample?
Plate 4
Under optimal conditions, which of the following grows fastest?
Bacteria
A bacteria with an initial population of 3 colonies, has a generation time of 20 minutes. How many bacteria will there be after 2 hours under optimum conditions for growth?
192
Which of the following are FAT-TOM factors that affect the growth of microorganisms?
Moisture, Texture, Oxygen, Time
Which of the following usually requires the highest pH (lowest acid level) for growth?
Bacteria
Which of the following terms describes a heat-loving organism?
Thermophile
The microorganism responsible for milk spoilage (while it is kept in the refrigerator) is
Psychrotroph
The term facultative anaerobic means an organism will grow under what type of conditions?
In the presence or absence of oxygen
Most spoilage bacteria require an Aw that is greater than _____?
0.91
A natural substance found in cranberries that inhibits the growth of yeasts and molds would be which of the following?
Sodium Benzoate
Which of the following inhibitory substances is added to bread to control the growth of mold?
Calcium Propionate
Under optimal conditions, which of the following grows fastest?
Bacteria
Which of the following is true of a bacterial endospore?
Are difficult to kill even with heat, Are very resistant structures, Is a survival mechanism of the organism
At the stationary phase, organisms are doing what?
Running out of food, space, etc.
Propionate inhibits ______________ while benzoate inhibits _____________.
Mold; yeast and mold
Plate count was used to determine the microbial load in 1 ml of milk. 35 colonies were counted on the 1:1000 dilution plate. How many CFU (Colony Forming Units) per ml were in the original sample of milk?
35,000
A bacteria with an initial population of 3 colonies, has a generation time of 20 min. How many bacteria will there be after 2 hours under optimum conditions for growth?
192
Patulin is a carcinogen produced by molds growing on corn.
False
In a restaurant food-prep area, mold is discovered on a 5 lb block of cheddar cheese. This should be carefully trimmed off before serving to customers.
False
St. Anthonys Fire, an epidemic during the Middle Ages, was the result of consuming moldy rye.
True
This is a potent liver carcinogen
Aflatoxin
There is a hypothesis that the strange behavior of the residents of Salem Massachusetts was the result of consuming mold-infested rye. What is the compound produced by the mold that could account for bizarre behaviors?
Lysergic acid
A highly resistant structure produced by only certain bacteria.
Endospore
Clostridium botulinum is a sporeforming bacterium that will not germinate at a pH...
<4.6
An emetic, such as the toxin produced by Bacillus cereus, causes
Vomiting
_________ causes a low body temperature and produces a heat-stable toxin
Staphylococcus aureus
__________ is an anaerobic bacterium that is heat-labile/sensitive and difficult to diagnose.
Clostridium botulinum
Which of the following is a medical use for Clostridium botulinum toxin
Clenching of jaw muscle, wrinkle remover
Honey is not safe for kids under 1 year of age, because Clostridium botulinum spores will be able to germinate, grow, and produce toxin in their intestinal track.
True
____________ is a facultative anaerobe that produces two different types of toxins.
Bacillus cereus
A foodborne intoxication is caused by the ________________, while a foodborne infection is caused by the presence of ________________.
Toxin; bacteria
What bacterium causes the most cases of SERIOUS foodborne illness?
Salmonella
What bacterium causes the MOST CASES of foodborne ILLNESS?
Campylobacter
This organism is found inside the egg of a chicken.
Salmonella enteriditis
Listeria is associated with
Lunch meat, stillbirths, unpasteurized milk
What pathogen(s) is/are associated with poultry?
Salmonella, Campylobacter
Consuming raw oysters will put you at risk of foodborne illness caused by _____________________.
Vibro vulnificus
Which of the following microorganism(s) is/are psychrotrophs (able to grow at low/refrigerator temperatures?
Listeria monocytogenes, Yersinia enterocolitica
A toxin-mediated infection is caused by
The toxin produced by the bacteria once it's growing inside of you
Which of the following causes toxin-mediated infections?
E. coli, Shigella
E. coli causes which of the following unique symtoms?
Bloody diarrhea, Hemorrhagic Colitis, HUS
__________ causes more gastrointestinal disease than any other organism and has been associated with outbreaks on cruise ships.
Norovirus
This organism is frequently involved in outbreaks in daycare centers.
Shigella
All E.coli strains are pathogenic, meaning that all E.coli strains are a human threat.
False
Which virus causes the MOST CASES of foodborne DISEASE?
Norovirus
Hepatitis A is the only hepatitis transmitted from food. It targets the
Liver
All of the viruses discussed in class are fecally transmitted.
True
Which of the following does NOT constitute a food as "potentially hazardous"?
High in lipids
Which of the following DOES constitute a food as "potentially hazardous"?
Low acid, High in protein, High Aw
Foods should be cooked at least to the minimum recommended temperatures. Those temperatures are...
Poultry: 165 F; Ground Meats: 155 F ; Other potentially hazardous foods: 145 F
The #1 parasite associated with humans is __________.
Giardia
This parasite has been associated with imported raspberries.
Cyclospora
__________ is associated with cats and can affect the unborn.
Toxoplasma
Pork is associated with __________.
Trichinella
Cause of a major waterborne outbreak in Milwaukee
Cryptosporidium
Tapeworm
Taenia
Reproduce by producing proglottids
Taenia
Associated with Sushi
Anisakis
Cats are a reservoir
Toxoplasma gondii
Single cell organism
Protozoa
Fermentation is a process of converting raw foods into fermented foods by using
Microorganisms
Which of the following is a good way to preserve meat?
Temperature (hot or cold), Salting, Drying, Smoking, Irradiation, Fermentation
All of the following are examples of fermented foods:
Cheese, beer, sausages, sauerkraut, bread
The types of microorganisms that are used in fermented food production include:
Bacteria, Yeasts, Molds
What is a probiotic?
Live bacteria that are good for your health, especially your digestive system
The main byproduct (waste) of fermented cheese manufacturing is
Whey
Which of the following are ways that microorganisms can be introduced into foods to initiate fermentation?
Add a culture containing desired organisms, Rely on indigenous organisms, Use of backslopping during production
How is the yeast removed from champagne after production is complete?
Frozen in a bath and pulled out through the top of the bottle
What is the function of the starter culture in sausage fermentation?
LABS produce lactic acid and lower pH, Produce flavor, Out-compete pathogenic microorganisms
Non-lactic acid bacteria can generally be inhibited and good bacteria can be selected for in fermented products by:
Adding salt, Creating anaerobic conditions (anaerobiosis), Lowering the pH
What antibody is associated with a true food allergy?
IgE
Anaphylaxis is a severe type of allergic reaction that involves two or more body systems (e.g., hives and difficulty breathing).
True
The "Big 8" are responsible for 90% of IgE-mediated food allergies and include all of the following EXCEPT:
Corn
__________ occurs when an individual lacks the enzymes necessary to utilize a specific food component. A classic example is lactose intolerance.
Metabolic food disorder
Which allergen does not cause the most food allergies in infants?
Crustacean shellfish
This food is responsible for most food allergy related fatalities.
Peanuts
This should be administered as soon as there is indication an individual is experiencing a severe allergic reaction.
Epinephrine
How does bacteria reproduce?
Binary Fission
What are the general characteristics of a virus?
1. don't replicate in food (not spoilage organisms)
2. are pathogens
3. only replicate in living cells
Spoils food, ellipsoidal shape, larger than bacteria, reproduce by budding
Characteristics of yeast
Grow by a network of filaments (mycelia), as these filaments get bigger, they form a sproangium that sends thousands of spores into the air
Process of growing for molds
Why is FAT-TOM important and what does it stand for?
Helps with controlling the growth of microorganisms in foods. Food Acid Temperature Time Oxygen Moisture
Between molds, yeasts, and bacteria, which grows at the lowest pH?
Molds
love heat
thermophiles
medium temp... room temp... body temp. Most pathogens are these
mesophiles
love cold
psychrophiles
tolerates cold, but grows better at room temperature (milk in fridge)
psychrotrophs
needs oxygen to survive
aerobic
can't grow in presence of oxygen (canned foods)
anaerobic