Pre Lab Quiz (Microscope)

Objectives part one

taking carefl notes with appropriate sections using the scientific method (table of content by week and page number)

practice aligment of cmp. light microscope and steromicroscope

sate micrometere to calibrate ocular scale

objective part two

usuing sizing ocular measure cellular structures

demonstarting digitial imagine skills by generating an image containing arrows and sizing bar

microscope aligment skills test

carryign the microscope

carry upright by the arm whiel supporting base

compound light microsope sample preparation

thin samples that light can pass through

select leaf, prepare epidermal peel

place with drop of water on slide

place coverslip avoiding bubbles

stereomicroscope

place sample using pennies on stage; illuminate and osberve

alighement of compound light microscope

repeat prodecure of producing goof image with new microsopes;

use digital camera to record images produced adn upload one image per slide as lab assiment on canvas

What to do for light microscope

turn on maximum power lamp

put auxilairy lens in upright position and raise condenser adn adust CD at setting 2

lower stage and rotate 10X

what to do for light microscope part two

move condenser up and down with weight adjustment knob to fous on iris diaphram

increase diameter with handle until just ousdie feild of view

remove one ocular lense and look won; openadn close condenser alot

test partners abilitey

higher power with light microscope

use fine focus at 40X

for eukarytoic total mag. is 400X

IF looking at bacterial cells

need 100X objectie to view cells

roate betewen 40X and 100X

use fine focus

calibrate your ocular micrometer using what

using the stage micrometer

to properly calicbrate your reticle with stage mucrometer you need to

align the zero line (beginning) of stage micrometer with zeor line or reticle

the eyepeice micrometer is divided into

100 units

(one onehundreth of an mm)