Adv. Hematology Unit 5 - Special Stains
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38 Terms
MPO stands for...
myeloperoxidase stain
What cells stain positive for MPO?
granulocytes (mature and immature)/monocytes (weakly)
What FAB classes of acute leukemia stain positive for MPO?
M0 (varies)/M1/M2/M3/M4/M5 (weakly)/M6
What does SBB stand for?
Sudan Black B stain
What cells stain positive for SBB
granulocytes (mature and immature)/monocytes (weakly)
What FAB classes of acute leukemia stain positive for SBB?
M0 (varies)/M1/M2/M3/M4/M5 (weakly)/M6
What does PAS stand for?
periodic acid Schiff stain
What cells stain positive for PAS?
lymphoblasts/lymphocytes/platelets and their precursors/erythoblasts/mature granulocytes/monocytes
What FAB classes of acute leukemia stain positive for PAS?
L1/L2/L3/M6/M7
What does SE stand for?
specific esterase
What cells stain positive for SE?
myeloblasts
What FAB classes of acute leukemia stain positive for SE?
M0 (varies)/M1/M2/M3/M4/M6
What cells stain positive for NSE?
monocytes (all stages)/erythroblasts/megakaryocytes
What FAB classes of acute leukemia stain positive for NSE?
M4/M5
What does TdT stand for?
Terminal deoxynucleotidyl transferase stain
What does NSE stand for?
non-specific esterase
What cells stain positive for TdT?
lymphoblasts
What FAB classes of acute leukemia stain positive for TdT?
L1/L2/L3
What does LAP stand for?
leukocyte alkaline phosphatase
LAP is used to differentiate ________ from ________
leukemoid reaction (increased)/CML (decreased)
What does TRAP stand for?
acid phosphotase with Tartrate resistance
What is TRAP used for?
diagnose hair cell leukemia
What kind of tube is used for an LAP test?
green top (heparin)
What element does MPO stain within cells?
myeloperoxidase
What element does SBB stain within cells?
lipids
What element does PAS stain within cells?
carbohydrates
What are two examples of substrates that are detected by an NSE stain?
naphthyl acetate/butyrate esterase
What element does SE stain within cells?
NASDCA
FAB class M0 leukemias stain positive for...
MPO (varies)/SBB (varies)/SE (varies)
FAB class M1 leukemias stain positive for...
MPO/SSB/SE
FAB class M2 leukemias stain positive for...
MPO/SSB/SE
FAB class M3 leukemias stain positive for...
MPO/SSB/SE
FAB class M4 leukemias stain positive for...
MPO/SSB/NSE/SE
FAB class M5 leukemias stain positive for...
MPO (weak)/SSB (weak)/NSE
FAB class M6 leukemias stain positive for...
MPO/SBB/PAS/SE/NSE
FAB class M7 leukemias stain positive for...
PAS/NSE
Cases of ALL stain positive for...
PAS/TDT
FAB class M4 leukemias stain positive for...