HEMA 1 LEC: LEUKOPOEISIS (NEUTROPHILS) P1

primitive stem cells

CFU-GEMM and CFU-L develop from_________ in the bone marrow before released into the circulation?

Polymorphonuclear cells or segments

In peripheral blood, neutrophils exist as?

Pluripotential hematopoietic stem cell

Stem cell pool consists of?

Mitotic pool (Proliferation)

Consists of progenitor cells

CFU-GEMM
CFU-GM
CFU-G

What are the progenitor cells in mitotic pool?

Type 1 Myeloblast

Identify

No visible granules

Type 2 Myeloblast

Identify

Contains <20 visible azurophilic granules

Granular blasts

Combining type 2 and 3 myeloblast is called?

IL-3, GM-CSF, G-CSF

Cytokines involved in neutrophil maturation?

Malignant acute promyelocytic leukemia

Absence of paranuclear halo is seen in?

Band

Identify

Secretory vesicles start to form

Integrins and Selectins

allow neutrophils to marginate and exit the blood to enter tge tissues (Diapedesis)?

CFU-GEMM and CFU-L


*Colony forming unit Granulocyte Erythrocyte Monocyte Macrophages
*Colony forming unit Lymphoid

What are the lineages of Leukocytes?

1. Light microscopy with Romanowsky stain
2. Flow cytometry through surface antigens

Leukocytes can be viewed into two methods?

Granulopoiesis

The production of neutrophils, eosinophils and/or basophils?

Stem cell pool

Consists of pluripotential hematopoietic stem cells?

CFU-G

What is the specific neutrophil progenitor?

Maturation pool (Storage)

Consist of metamyelocyte, band neutrophils, and segmented neutrophils?

metamyelocyte, band neutrophils, and segmented neutrophils

Maturation pool consists of?

14-20um

Myeloblast size?

8:1 to 4:1

Myeloblast N:C ratio?

Myeloblast

Identify

The shape is round to oval

Myeloblast

Identify

The nucleolus is 2-4

Myeloblast

Identify

Staining is slightly basophilic

Myeloblast

Identify

Population is 0-3%

15 hours

Myeloblast stage lasts approximately?

Type 3 Myeloblast

Identify

Darker chromatin

Type 3 Myeloblast

Identify

Purple cytoplasm

Type 3 Myeloblast

Identify

>20 visible azurophilic granules

Type 3 Myeloblast

Identify

Rare in normal bone marrow

Acute myeloid leukemia

Type 3 Myeloblast seen in what condition?

16-25um

Size of
Promyelocyte?

3:1 to 2:1

N:C ratio of Promyelocyte?

Promyelocyte

Identify

The shape is round to oval, eccentric

Promyelocyte

Identify

Heterochromatin, slightly course

Promyelocyte

Identify

Nucleolus is 1-3

Promyelocyte

Identify

The staining is basophilic

Azurophilic

What is the granules in
Promyelocyte?

15 hours

Promyelocyte last stage is approximately?

Paranuclear halo or "hof "

Is seen around the nucleus in normal cells?

Promyelocyte

Identify

Chromatin clumping is visible around the nucleus

12-18um

Size of myelocyte

1:!

N:C ratio of
Myelocyte?

Myelocyte

Identify

Chromatin is coarse and clumped

Myelocyte
Metamyelocyte
Band

Identify

Nucleolus is none

Myelocyte

Identify

Staining is basophilic and acidophilic

Dawn of Neutrophilia

Myelocyte is also known as?

affinity for neutral stains

Mature cells are able to called neutrophil because they have?

4.3 days

Myelocyte stage lasts?

Myelocyte

Identify

Production of primary granules stops

Myelocyte

Identify

Formation of secondary granules

Myelocyte

Identify

Last stage of mitosis

Patches of grainy pale pink cytoplasm

Myelocyte Early stage is similar to promyelocyte but they contain?

Myelocyte Early stage

Identify

Nucleus are heterochromatin

15-18um

Size of
Metamyelocyte?

1:1

N:C ratio of
Metamyelocyte

Metamyelocyte

Identify

Kidney-shaped, peanut-shaped

Metamyelocyte

Identiy

Chromatin is coarse and clump

Metamyelocyte
Band

Identify

Staining is beige and salmon

3-10%

Metamyelocyte population?

Tertiary granules (Gelatinase granules)

Metamyelocyte synthesize what granules?

Tertiary granules (Gelatinase granules)

responsible for locomotion?

Metamyelocyte

Identify

Cytoplasm contains residual RNA

9-15um

Size of Band?

1:1-1:2

N:C ratio of band?

C-shaped, S-shaped

Shape of Band?

Band

Identify

Chromatin is coarse, clump

Marrow: 9-32%
Peripheral: 0-5%

Population of
Band?

Band

Identify

RNA is absent?

Band

Identify

Tertiary granules are still being formed

Segmented neutrophils

Identify

Secretory granules continue to form

Presence of 2-5 lobes connected by thread-like filament

What is the major difference against band neutrophils and segmented neutrophils?

MAC-1

Trigger the death and phagocytosis of neutrophils?