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(LIS)laboratory information system
computer system integrating every part of the labatory testing process from orders through analysis and reports
Accession number
Number generated by laboratory information system (LIS) when specimen request is entered into the computer
how can you use the accession number?
can be used to trace specimens and results through the LIS at each stage f process
how can LIS help the process of blood collection?
1- deduce the chance of identification errors in patient care
2- PBT must take care they're entering info correctly
what info is on all requisition papers?
1- patients name
2- patient DOB
3- gender
4- name and contact info of health care provider
5- tests ordered ordered and Amy special requirements such as fasting , timed collection etc
6 also may include diagnosis codes, phone and address, hospital record number, insurance info
PBT mantra
I'm only here to draw blood you'll have to discuss your questions with your dr
why are blood tests ordered?
1- part of routine care
2- aid in diagnosis
3- to monitor specific medication or condition
provider
provides care ex dr or clinic
payer
pays med bill ex medicare or medicare
Analyte
Substance being measured in a diagnostic test
most lab tests require ...........blood
veinous
specimens are managed in different ways depending on.......
the analyte involved
CBC (complete blood count)
a set of tests that include all WBC, RBC and platelet measurements.
ESR (erythrocyte sedimentation rate)
The rate in which it takes for RBC's to settle off in an unclotted tube. Normal rate is 1 ml in 5 min.
what do blood cultures detect?
bacterial or fungal infections in patients blood
Electrolytes are
substances that affect the flow of nutrients and the removal or waste products in blood
name examples of analytes?
electrolytes, enzymes, proteins, antibodies, vitamins and minerals
test panel is a group of test that either
1- related to analytes ex electrolyte panel or lipid panel
2- related to a unifying condition ex hepatic panel (liver function test)
renal panel
kidney function test
hepatic panel
liver function test
basic metabolic panel
group of eight specific blood tests that provide important information about the current status of the patient's kidneys, electrolyte balance, blood sugar, and calcium levels
what tube for basic metabolic panel
SST or heparin tube
fasting sometimes requested
what's specifically tested for in BMP?
Glucose
calcium
sodium
potassium
carbon dioxide
chloride
blood urea
creatinine
BUN
blood urea nitrogen
waste product related to kidney function
Creatinine
waste product of muscle metabolism, filtered out of the blood by the kidneys, and excreted in urine
examples of panel tests
1- basic metabolic panel
2- comprehensive metabolic panel
3-lipid panel
4- CBC
comprehensive metabolic panel (CMP)
tests in addition to basic metabolic panel for expanded screening purposes: albumin, bilirubin, alkaline phosphatase, protein, ALT, and AST
what tubes for CMP?
SST, heparin(may be stat), sometimes fasting
lipid panel
series of blood tests (total cholesterol, high-density lipoprotein, low-density lipoprotein, and triglycerides) used to assess risk factors of ischemic heart disease
what tube for lipid panel?
SST, heparin(may be stat), usually fasting
complete bood count
common blood test to determine number of RBC, WBC and platelets in blood
CBC with differential
number of RBC and WBC and platelets and specific number of each type of WBC
what tube for CBC?
EDTA
be gentle
why use a tourniquet ?
to aid in locating the best site for venipuncture
where to use tourniquet?
3-4 inches above puncture site
when using a BP cuff as tourniquet how much do you inflate?
just below diastolic or no higher than 40 hg
why place tourniquet over clothing or on top of gauze?
to protect thin or fragile skin
Antecubital
anterior surface of elbow where blood is usually drawn
how long can you leave tourniquet on?
no longer than 1 min bc it will cause pain or mess up test
Hemoconcentration
a build up of blood cells (solid)relative to the liquid components of blood
what causes hemoconcentration?
leaving tourniquet on too long and it falsely elevates the blood cell count ....changes blood count
hemolysis
destruction of red blood cells
what causes hemolysis?
leaving tourniquet on too long
what if you leave tourniquet on over 1 min?
must wait 2 min b4 trying again
order of the end of a blood draw?
remove
1- tourniquet
2- tube (inversions)
3- needle
tourniquet makes blood pool and .............
makes veins easier to see
lactate is so sensitive to tourniquet use.......
some facilities dictate not using one when specimen tested for lactate
antecubital fossa
inside of elbow
what vein is PBT first choice?
median cubital
why is median cubital best vein choice ?
it's well anchored
using it has least chance of accidentally hitting a nerve or artery
2 kinds of vein formation?
M and H
veins in order of perference?
1- median cubital
2- cephalic
3- basilic
median cubital also known as
median basilic
the primary consideration for PBT to choose a venipuncture site?
the patients safety which means avoiding nerves and arteries
avoid veins that are hard to see or palpate
least favorable vein?
basilic bc near brachial artery and ante brachial cutaneous nerves
most reliable way to choose a vein?
palpation
when palpating veins consider the following factors?
1- vein health
2- vein depth
3- anchoring of vein
4- diameter of vein
vein health?
vein should be springy and uniform not hard or bumpy
vein depth?
veins may not be seen on obese people. no matter of the depth of vein the angle is same
might need longer needle
anchoring of the vein?
meaning they don't roll. palpation helps determine rolling
diameter of vein?
helps determine gauge of needle
water encouraged in both fasting and not fasting draws
water
when vein difficult to locate?
1- hang Arm straight down causing blood to pool
2- warm cloth or heat pack veins dilate
3- make fist will cause blood to surge
4- do not pump repeatedly can cause hemoconcentration or hemolysis
5- ask where has been successful b4
6- never slap veins
if no arm veins suitable then use
back of hand
dorsal aspect
back of hand
never use veins in wrist bc of
nerves, tendons and arteries
if no veins in arms or hand then try
lower leg , ankle or foot
draw sites in lower limbs are elevated risk for
thrombosis
thrombosis
blood clot
dr orders
leg, ankle, and foot draws need dr approval
what is first priority for PBT when selecting a site?
safety and health. select a site with least discomfort
clean site?
70% alcohol in circles starting inside working out and let dry
how to clean for blood culture?
most common chloraprep
can use alcohol but 3 pads for 60 sec
if iodine then clean off with alcohol so no trace
iodine allergy
shellfish
when testing for alcohol level how to clean site?
use something other than alcohol or iodine so not to contaminate blood
how to anchor vein?
use thumb to anchor vein pulling skin below site
info about inserting needle
bevel up
30% angle
"Blown" Veins
needle thru vein
what problems can prevent blood flow?
1- vein have moved or rolled
2- needle gone thru vein
3- bevel not fully inserted into vein or vein collapsed
do not probe for vein, if blood isn't immediate you might create a
hematoma
Hematoma
leaked blood beneath skin
PBT can move needle ........... but never .........
forward and back
side to side
if you cannot get blood from a site what do you do?
remove tourniquet , remove needle ans select another site
how many tries to stick?
2 attempts then get a supervisor
describe how most venipuncture are done?
in antecubital area of arm with a straight 21 gauge multisample needle in evacuated tubes
what's so great about multisample needles?
easier to handle and pose less risk of accidental needle stick
what to ask patient b4 stick?
1- have you had difficulties drawing blood b4
2- have you met the fasting requirements
3- no food or gum
what equipment is needed when performing routine venipuncture with multisample needle and evacuated tubes?
1- requisition
2- soap water sanitizer
3- gloves or PPE
4- tourquinet
5- 70% alcohol
6- multisample needle
7- needle holder
8- collection tubes
9- specimen labels
10- 2 gauze
11- bandage
12-pen
Discard tube
also called a clear tube, used to collect and discard approximately 5mL of blood to prevent IV or tissue fluid contamination of a specimen
must be a citrate tube or no additive
Butterfly needle
a small, winged-tip needle used to initiate intravenous access
higher risk for needle stick
what tubes to use for elderly or pediatric ?
smaller tubes bc put less pull on veins
how to treat pediatric patients?
1- appropriate equipment
2- appropriate vocabulary
3- get on physical level
when pediatrics in hospital setting getting blood drawn they have an increased chance of iatrogenic anemia which is
anemia resulting from excessive blood removal
no more than ........... of patients blood should be drawn in a day
2.5 %
no more than ......... of patients blood should be drawn in a 30 day period
5%
how to treat obese patients when trying to draw blood?
1- vein location hard bc veins deeper
2- allow arm to dangle
3- apply warm pack
4- ask patient about a successful site used before
5- bp cuff inflated to diastolic
6- still use 30 degree angle
7- anchor veins firmly
8- only two attempts
when dealing with patient with cognitive impairment or mental health disorder?
1- patient behavior unpredictable
2- keep supplies out of reach
3- have another staff member in room
4-have family member there
5- be prepared to remove needle and tourniquet quickly
when dealing with patients having a seizure?
1- if seizure remove tourniquet and needle and discard
2- keep patient safe from head injury
3- protect patient and PBT from needle stick
syncope
fainting
how to handle fainting during a blood draw?
1- very common
2- no warning
3- patients usually inform you if it happens often
4- should recline if possible
5- if fainting release tourniquet and needle
6- keep patient safe
7- get help
8- if feeling faint b4 stick use cold cloth, rest juice or snack