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enzyme (protein level & definition/use)
tertiary protein level; is an organic catalyst and lowers activation energy of chemical reactions
denaturation
an enzyme loses its physical when its bonds break, and it can no longer bond with its corresponding substrate (it loses its function)
enzyme specifity
each unique chemical reaction has its own enzyme, and it has to physically fit into the active site of the enzyme in order for the enzyme to work
lock and key theory
only one substrate can fit into an enzyme’s active site and cause a chemical reaction
Induced fit model
the enzyme changes form slightly to lock the substrate in the active site before the chemical reaction occurs, and reverses the shift, releasing it after the reaction
optimal temperature for enzymes to work
40 degrees celsius
what happens when temp is over/under 40 degrees?
over: enzymes start to denature, lowering the reaction rate until the reaction completely stops
under: the molecules have less energy and therefore less collisions occur between the substrate and enzymes, lowering the reaction rate
enzymes and their relationship w pH
each enzyme has its unique optimal pH, and denatures if it is not at that pH
point of saturation
when the amount of substrate increases, at one point the rate of reaction will remain the same as all the enzymes are constantly bonding with a substrate; the enzymes are saturated and the rate of reaction won’t increase unless more enzymes are added
enzyme concentration
if you hate a set amount of enzymes, the reaction rate will increase the more enzymes you add until they use up all the substrate, ending the reaction very quickly
heavy metals
heavy metals like mercury, cadmium, or lead can denature enzymes
coenzyme traits
non-protein organic molecule that is composed of vitamins → does not denature
coenzyme functions
used as a helped molecule in a reaction, carrying smaller molecules to the next reaction so it does not get lost
competitive inhibitors
are bad and not supposed to be in the body!!!!
irreversible inhibitors
permanently blocks the enzyme and there is no chemical reaction
reversible inhibitor
inhibitor bonds with enzyme and creates a reaction, producing a product (the PRODUCT of the reaction is poisonous, not the inhibitor)
non competitive inhibitor
naturally occurring, inhibitor attaches to enzyme at a site other than active site, causing the enzyme to denature (allosteric interaction) → shuts down metabolic pathways in the body
equation for photosynthesis
2CO2+2H2O+light energy → C6H12O6+6O2
light reaction equation
light energy + water → NADPH+ATP
Non cyclic phosphorolation creates:
NADPH and ATP
Cyclic phosphorolation creates:
ATP only
photolysis does what….!
splits an H2O atom for its electrons
chemiosmosis does what
produces ATP as the ATP synthase spins and adds energy into the ADP bonds
calvin cycle three stages
carbon fixation
reduction
regeneration of RuBP
Calvin cycle equation
CO2+NADPH+ATP+RuBP → RuBP+G3P+ADP+NADP
what happens in carbon fixation
RuBP attaches to CO2 through an enzyme called rubisco
what happens in reduction
energy transfer: ATP and NADPH unload energy onto the 3-carbon molecs → G3P
what happens in regeneration of RuBP
1 G3P leaves cycle, the other 5 are used to regenerate 1 molecule of RuBP for the next cycle
how many molecules of G3P are used to turn into glucose?
2
C-3 plant
typical plants → stomata is open most of the time and can easily do light reaction and the calvin cycle in the mesophyll cells (cannot store CO2)
C4 plants
live in hot, dry environments → stomata is not opened all day, so CO2 can be stored as a 4 carbon organic compound to be used later (the calvin cycle occurs in the bundle sheath cells so the C-4 compound can release stored CO2) → spatial separation
CAM plants
live in hot, dry environments → stomata opens and carbon fixation occurs at night and the resulting organic acids are stored in the mesophyll cells → the calvin cycle occurs during the day (temporal separation: calvin cycle and carbon fixation occur at diff times of day but both in the mesophyll cells)
when does photorespiration occur
when plants close their stomata, they cannot release oxygen nor replace used CO2
why is photorespiration bad?
oxygen is a better competitor for RuBP and will attach to the RuBP, leaving no space for CO2 → less glucose is produced as carbon fixation cannot occur
substrate level phosphorylation
ATP directly produced from glycolysis and the citric acid cycle
oxidative phosphorylation
indirect energy conversion from coenzymes into ATP