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Operon
Series of protein-coding regions in bacteria under the control of a single regulatory region
Regulatory region followed by multiple coding regions
Lac operon purpose
Breaks down lactose
Parts of lac operon
Promoter
Operator
lacZ
lacY
lacA
LacY protein
Channel protein, allows lactose into cell
LacZ
Enzyme that breaks down lactose into glucose and galactose
LacI
transcription factor (repressor)
lac operon in absence of lactose
Lac operon is blocked by LacI repressor
lac operon in presence of lactose
LacI binds to allolactose and loses its affinity for DNA
basal expression
In absence of LacI repression, the operon is transcribed at low levels