BI157L - Chlorophyll + Fruit Flies Exam Review

Pixel values range from 0 to ____?

255

Which of the following statistical tests can be used to compare more than two variable groups?

ANOVA

In a greyscale image, if the pixel value is 1 that means the the pixel color is ______________?

a dark grey

Which wavelengths of light does a plant absorb when converting sunlight into energy?

red and blue

Which wavelengths of light does a plant reflect when converting sunlight into energy?

green

What was the purpose of the "split channel" function in ImageJ?

to split the image into red, green and blue components; so that we could quantify the value of green in the pixels

During our plant experiment: What was the dependent variable, or the outcome that we intend to measure at the end of the experiment?

% greenness

During Module 3 (Our plant experiment): What was the independent variable(s), or the factors that we purposely control and manipulate in order to effect the outcome of the experiment?

light condition, soil, seed type

You notice two colors of flowers growing outside of your house, about half of them are red and half of them are blue. You wonder if both colors of flowers typically grow to the same height, or if the growth habits differ depending on the color of flower. You make the following hypotheses:

Hypothesis 1: On average, the red flowers will have a significantly greater height than the blue flowers.

Null Hypothesis: On average, the red and blue flowers are the same height.

Which of the following should you use to test this hypothesis?

One-tailed T test

Which of the following is used to eradicate certain insects?

Sevin

What are the independent variable(s) in our fruit fly experiment? Aka, the variables that we included and manipulated in order to test if they have an effect on the outcome?

herbicide concentration,

insecticide concentration

What is the outcome/dependent variable in our fruit fly experiment, aka, the thing that we will measure to find out the result of our experiment?

# fruit fly adults,

# fruit fly pupa,

# fruit fly larva

What was the dependent variable in our chlorophyll experiment? Aka, the thing that we measured at the end to find out the results of our experiment?

% greenness

For what purpose did we use Ethanol? (EtOH)

To extract chlorophyll

What is Sevin used for?

Pesticide

What was the research objective of the chlorophyll experiment?

to analyze the percent greenness of two different seed types, in two different soil types, and

placed in varying conditions

True or false: You can use a t-test to compare the averages of two or more variable groups

False

True or false: You can use an ANOVA to compare the averages of two or more variable groups

False

What code do you need to type in in R before importing your data?

library(Rcmdr)

How do you import data in R?

Data -> Import Data -> From text file, clipboard, or URL

How do you do a Multiway ANOVA in R?

Statistics -> Means -> Multiway ANOVA

What do you choose for factors? For response variable?

atrazine.concentration, sevin.concentration; larvae

If there are no main effects and no interactions, ________________________________.?

we do not need to graph anything

Because there is a main effect AND an interaction, _______________________________.?

we only graph the interaction

What do the ":" represent?

interactions

What two characteristics does a female and male fruit fly have in common?

sternite and anal plate

The closer an image's pixel value is to 0, _____________________?

the more black it is

The closer an image's pixel value is to 255, _____________________?

the more white it is

The darker a pixel is, ____________________?

the more green it is

The closer the pixel value is to 0, ___________________?

the more green it is

How do you find percent greenness?

Mean/255*100