Chapter 2 Bio-Rad Biotechnology

Danger to the eyes exist. Safety goggles should be worn

Eye Safety

Misuse of chemicals could cause explosion

Explosion Safety

Poisonous substances are present

Poison Safety

Using live animals. Safety of animals should be ensured

Animal Safety

Danger of cuts or punctures caused by use of sharp objects

Sharp Object Safety

Radioactive materials are used

Radioactive Safety

Chemicals used can cause burns or are poisonous if absorbed through skin

Chemical Safety

Care should be taken around open flames

Fire Safety

Substances used could stain or burn clothing

Clothing Protection Safety

Chemicals or chemical reactions could cause dangerous fumes

Fume Safety

Use caution when handling hot or extremely cold objects

Thermal Safety

Care should be taken when using electrical equipment

Electrical Safety

Poisonous plants or plants with thorns are to be handled

Plant Safety

Danger involving Bacteria, Fungi, or Protists

Biological Hazzard

Use of open flame could cause fire or explosion

Open Flame Alert

Care must be taken to dispose of materials properly

Disposal Alert

National Fire Protection Association (NFPA)

establishes codes, standards, guidelines, and recommended practices for the prevention and control of fire

Health (NFPA)

blue

Fire Hazard (NFPA Label)

red

Flammibility (NFPA)

yellow

BSL-1

no special precautions; basic teaching labs (E. coli)

BSL-2

Indigenous microorganisms that can lead to diseases of varying severity in healthy adults.

Dillution formula

C1V1=C2V2

Molarity formula

moles of solute/liters of solution

minuscus

is the curve that you see at the liquids surface

Micropipette

A laboratory instrument used to measure, dispense, and transfer very small amounts of liquid.

serological pipette

Used to measure and deliver large volumes of liquids.

disposable pipet

disposable object used to transfer small volumes of liquid

volumetric flask

for making up solutions to a known volume

weight boat

Used to weigh or transfer chemicals (usually dry)

on a mass scale

scale used to measure mass

Beaker

used to hold liquids

Erylenmeyer Flask

better than beaker for mixing, so substance doesn't splash

graduated cylinder

instrument used to measure volume of a liquid

lab notebook

notebook for recording information important to a study, such as design, procedure, and the planned analysis

Standard Operating Procedures (SOPs)

specific sets of written instructions about how to perform a certain aspect of a task

percent error formula

experimental value-accepted value/accepted value x 100

Titration

a measured amount of a solution of unknown concentration is added to a known volume of a second solution until the reaction between them is just complete

molar mass

the mass of one mole of a pure substance

Chemical Label

3 parts to a label (ID of Chemical, Date and Initials of Who made the chemical)