lab unit 2 quiz 1 Exoenzymes- Skim Milk Agar, Tributyrin Agar, Gelatin, Starch Agar, DNA Agar, Carbohydrate Fermentation

can do writing

Name an example of an exoenzyme.

Amylase

     Where do exoenzymes function?

Exoenzymes work outside the cell in the environment

 What is the SUBSTANCE that is modified by an exoenzyme?

substrate

the SUBSTANCE that is modified by an exoenzyme example

substrate

Amylase breaks down starch into smaller sugars

  What type of reactions are controlled by exoenzymes?

catabolic reactions .

   What type of MEDIA are the exoenzyme media used in this lab?

Differential media

 What MOLECULE gives skim milk agar its white color?

casein protein

  What is the SOLIDIFYING AGENT in nutrient gelatin?

collagen protein

What REAGENT is used to evaluate the results of the starch reaction?

iodine

What SUBSTANCE does the reagent used in the starch reaction test for the presence of?

The reagent iodine tests for the presence of starch

What color is the DNA agar?

Green

What is the pH INDICATOR in the carbohydrate fermentation broth?

phenol red

 What is the name of the small tube that is upside down in the carbohydrate fermentation tube?

Durham tube

What is the function of the small tube that is upside down in the carbohydrate fermentation tube?

indicates if gas is produced
bubble in Durham tube- gas
no bubble in Durham tube- no gas

skim milk agar (substrate)

Casein

skim milk agar (enzyme)

Casease

skim milk agar (product)

Amino acids

skim milk agar (positive result)

Clear zone around colony

skim milk agar (positive bacteria)

Bacillus

tributyrin agar (substrate)

Triglycerides

tributyrin agar (enzyme)

Lipase

tributyrin agar (product)

Glycerol + Fatty acids

tributyrin agar (positive result)

Clear zone around colony

tributyrin agar (positive bacteria)

enterococcus

starch agar (Substrate)

Starch

starch agar (Enzyme)

Amylase

starch agar (Product)

Maltose

starch agar (Positive Result:)

purple with iodine

starch agar (Positive Bacteria)

Bacillus subtilis

DNA agar (Substrate)

DNA

DNA agar (Enzyme)

deoxyribonuclease

DNA agar (Product)

Nucleotides

DNA agar (Positive Result)

Clear zone around colony

DNA agar (Positive Bacteria:)

Serratia

nutrient gelatin (Substrate)

Collagen

nutrient gelatin (Enzyme)

Collagenase

nutrient gelatin (Product)

Amino acids

nutrient gelatin (Positive Result)

becomes liquid

nutrient gelatin (Positive Bacteria)

Bacillus

Carbohydrate Fermentation (Phenol Red Broth)-Substrate

Glucose, lactose, or sucrose

Carbohydrate Fermentation (Phenol Red Broth)

Enzyme

not specified

Carbohydrate Fermentation (Phenol Red Broth)

Product

acid fermentation, alkaline fermentation, or gas

Carbohydrate Fermentation (Phenol Red Broth)- Positive Result:

Color change of broth

• Acid: Yellow

• Alkaline: Red