DNA Sequencing - Dideoxy/Sanger Method

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Last updated 3:16 AM on 6/26/26
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5 Terms

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DNA Template

Segment of interest for determination

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DNA Primer

A short, single-stranded oligonucleotide that binds to the template strand to provide a starting point for DNA synthesis

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DNA Polymerase

An enzyme (such as Sequenase or Taq polymerase) that synthesizes the new DNA strand by adding nucleotides complementary to the template

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Deoxynucleoside Triphosphates (dNTPs)

Normal, unlabelled nucleotides (dATP, dTTP, dCTP, dGTP) that serve as the building blocks for extending the growing DNA chain

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Dideoxynucleoside Triphosphates (ddNTPs)

Modified versions of the normal nucleotides (ddATP, ddTTP, ddCTP, ddGTP) that lack a 3'-hydroxyl (\(-OH\)) group. This missing group prevents the next nucleotide from attaching, causing chain elongation to stop