Pseudomonas aeruginosa

P. aeruginosa general characteristics

Gram (-) rods

Motile

Obligate Aerobe

Nosocomial pathogen

NOT FASTIDIOUS - minimal nutritional requirements

P. aeruginosa produces what type of odor

Sweet/Grapelike/corn taco

P. aeruginosa antigenic structures

Pili

Exopolysaccharide

Lipopolysaccharide

Elastases

Exotoxin A

P. aeruginosa maximum tolerated temp

42°C

P. aeruginosa basic forms

Free swimming single cell (single flagellum)

Biofilm

P. aeruginosa virulence factors

Slime layer - biofilm

Single polar flagellum

LPS

Extracellular enzymes

Pili

Pigments

P. aeruginosa extracellular enzymes

Protease

Elastase

Alkaline protease

Lecithinase

Phosphorylase C

Exotoxin A

Exotoxin S

P. aeruginosa pigments

Pyocyanin - destroy other microbes for nutrients

Pyoverdin - fluorescent pigment

Pyochelin - serve as siderophore (for getting iron)

P. aeruginosa pili need what to attach

Host cell injury

Exotoxin A fxn

Blocks protein synthesis

Antiphagocytic

Exo-enzyme S fxn

Adhesins to glycolipid of respi cells

Produced in burn tissues

Phosphorylase C fxn

Attack pulmo cells producing atelectasis and necrosis

Alk protease fxn

Lyses fibrin

Responsible for hemorrhagic skin lesion, corneal infection

Elastase fxn

Degrades elastic fibers in alveolar wall

Degrades the ff.

• IgG

• IgA

• Complement

• Collagen

• Coagulation factor

P. aeruginosa clinical manifestations

Swimmer’s ear / External otitis

Meningitis

Bacteremia

Ecthyema gangrenosum - sepsis on neonate w severe burn lesion

Hot tub folliculitis

Green nail syndrome

P. aeruginosa diagnosis and culture

BAP 42°C

MAC - non lactose fermenter

Isolation agar: pyocyanin blue-green

Fruity odor

Oxidase +

Fluorescent under UV light

P. aeruginosa AMR plasmid

R-factor plasmid

P. aeruginosa is resistant to

Penicillin

Kanamycin

Tetracycline

P. aeruginosa is sensitive to

Aminoglycosides

• Amikacin

• Gentamycin

• Tobramycin