EXERCISE 1: INTRODUCTION TO MICROSCOPY

0.0(0)
studied byStudied by 0 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/55

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

56 Terms

1
New cards

Microscopy

technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye

2
New cards

Microscope

optical instrument used to view or magnify minute specimens

3
New cards

Microscope

an instrument that produces enlarged images of small objects

4
New cards

Illumination of the Specimen

a light source (LED, halogen bulb, natural light) is directed towards the specimen light passes through a condenser lens (directs light onto the specimen)

5
New cards

Passage of Light through the Specimen

light can be absorbed, transmitted, or refracted, creating an initial image

6
New cards

Objective Lens Magnification

light passes through the specimen and enters the objective lens (responsible for the initial magnification of the image)

7
New cards

Formation of the Real Image

objective lens produces a real, inverted image of the specimen, which is then projected into the body tube of the microscope

8
New cards

Ocular Lens (Eyepiece) Magnification

real image formed by the objective lens is further magnified by the eyepiece, which is another convex lens

9
New cards

Formation of the Virtual Image

eyepiece magnifies the real image into a larger virtual image that appears to be located at some distance away from the eyepiece

10
New cards

Magnification

degree to which an object is enlarged when viewed through a microscope

11
New cards

1,000 times the actual size of the specimen

how many times can a light microscope magnify?

12
New cards

Resolution

degree of clarity of an image when viewed through a microscope

13
New cards

Contrast

distinction between the light intensity of the specimen image and the adjacent background relative to the overall background intensity

14
New cards

Robert Hooke

first to identify and name a cell through a study of cork under a microscope

15
New cards

Cells

coined this term because they resembled the small rooms occupied by monks

16
New cards

Simple Microscope (Magnifier)

Light Illuminated, Single lens system, The image appears in 3D, Individual cells cannot be seen due to its low magnification, Enlarges objects without inverting it

17
New cards

Compound Microscope

Light Illuminated, The image appears in 2D, The most commonly used microscope, Individual cells, even living cells, can be viewed, Has high magnification but low resolution

18
New cards

Stereoscopic/Dissecting Microscope

Consists of 2 microscopes mounted in one body, Each ocular (eyepiece) can be adjusted, Low magnification, Has a 3D perspective, Used to view live specimen

19
New cards

Transmission Electron Microscope (TEM)

Electron Illuminated, Image is seen in 2D, but its picture appears in Black & White, Has high magnification and resolution

20
New cards

Scanning Electron Microscope (SEM)

Electron Illuminated, Image is seen in 3D, Has high magnification and resolution, but the image appears in Black & White, Specimen is coated in gold, and the electron bounces off to give you an exterior view of the specimen

21
New cards

Base, Pillar, Arm, Inclination Joint, Stage, Stage Clip, Body Tube, Draw Tube, Dust Shield, Revolving Nosepiece, Adjustment Knob, Coarse Adjustment Knob, Coarse Adjustment Knob, Fine Adjustment Knob

Mechanical Parts

22
New cards

Base

V or U-shaped structure that supports the whole instrument

23
New cards

Pillar

vertical extension of the base to which the arm is attached

24
New cards

Arm

curved basic part of the microscope to which the base, body, and stage are attached

25
New cards

Inclination Joint

Moveable part that facilitates the tilting of the microscope

26
New cards

Stage

platform upon which the slide containing the specimen is placed

27
New cards

Stage Clip

pair of metal parts that holds the slide in place

28
New cards

Body Tube

hollow cylinder in front of the upper part of the arm that serves as a housing for the lens; Serves as a passageway of light from the objectives to the eyepiece

29
New cards

Draw Tube

smaller cylinder attached to the base of the body tube that holds the ocular

30
New cards

Dust Sheild

fixed plate attached to the base of the body tube & situated above the revolving nosepiece; Protects objectives from dust and dirt

31
New cards

Revolving Nosepiece

rotary head is attached to the base of the body tube and holds the objective; facilitates the shifting of the objective

32
New cards

Adjustment Knob

used to adjust the objective when focusing, which, when turned clockwise or counter-clockwise, lowers or raises the body tube

33
New cards

Coarse Adjustment Knob

upper, larger knobs used for faster movement of the body tube when focusing on the lower power objective

34
New cards

Fine Adjustment Knob

lower, smaller knobs used for final focusing under high power objectives and in viewing at different levels

35
New cards

Abbe Condenser (Illuminator), Iris Diaphragm, Mirror

illuminating parts

36
New cards

Abbe Condenser (Illuminator)

lowers or raises the intensity of light

37
New cards

Iris Diaphragm

plate that regulates the amount of light that enters the condenser

38
New cards

Mirror

Usually 2 faces where one surface is Plane (for a very bright source of light) and the other is Concave (for low intensity of light). Used to reflect light through the objective lens and into the eyes

39
New cards

Eyepiece/Ocular, Objectives (Scanner, LPO, HPO, Oil Immersion Objective)

Magnifying Parts

40
New cards

Eyepiece/Ocular

detachable tube on top of the draw tube. May be provided with a pointer, which is used to point to a part of the specimen

41
New cards

Scanner (Red)

shortest cylinder with the widest opening; lowest magnification; used to observe a wider field of objects (4x-5x)

42
New cards

Low Power Objective/ LPO (Yellow)

smaller lens opening; observing the general outline of the object under study and locating various parts of the specimen (10x)

43
New cards

High Power Objective/HPO (Blue)

longer tube with smaller lens compared to LPO (40x-50x)

44
New cards

Oil Immersion Objective (White)

longest tube with the smallest opening (90x-100x)

45
New cards

Eyepiece/Ocular

A.

<p>A.</p>
46
New cards

Arm

B.

<p>B.</p>
47
New cards

Rack Stop

C.

<p>C.</p>
48
New cards

Stage Clip

D.

<p>D.</p>
49
New cards

Coarse Adjustment Knob

E.

<p>E.</p>
50
New cards

Fine Adjustment Knob

F.

<p>F.</p>
51
New cards

Revolving Nosepiece

G.

<p>G.</p>
52
New cards

Objective Lens

H.

<p>H.</p>
53
New cards

Stage

I.

<p>I.</p>
54
New cards

Condenser

J.

<p>J.</p>
55
New cards

Illuminator

K.

<p>K.</p>
56
New cards

Base

L.

<p>L.</p>