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Prokaryotes
Primitive cells with a simple structure that lack membrane-bound organelles; e.g. bacteria.
Eukaryotes
More complex cells that contain membrane-bound organelles; e.g. cells that make up animals and plants.
Mitochondria
Organelles that produce energy in the form of ATP through respiration.
Chloroplast
Organelle found in plants where photosynthesis takes place.
Cell Membrane
The outer boundary of the cell that controls the passage of substances in and out.
Isotonic solution
A solution with the same solute concentration as the inside of the cell.
Hypotonic solution
A solution with a lower solute concentration than that inside the cell.
Hypertonic solution
A solution with a higher solute concentration than that inside the cell.
Diffusion
The movement of molecules from an area of high concentration to an area of low concentration.
Osmosis
The diffusion of water across a selectively permeable membrane.
Facilitated Diffusion
The process by which molecules move through channel proteins in the cell membrane.
Concentration Gradient
The difference in concentration of a substance across a membrane.
Surface Area to Volume Ratio
A measure used to compare the surface area of a cell to its volume, affecting material exchange.
Rough ER
Endoplasmic reticulum with ribosomes that makes and modifies proteins.
Smooth ER
Endoplasmic reticulum that synthesizes lipids and detoxifies certain chemicals.
Ribosomes
Cell organelles made of RNA and protein that synthesize proteins.
Lysosomes
Membrane-bound vesicles containing digestive enzymes that break down waste.
Golgi Body
An organelle that processes and packages substances made in the cell.
Prokaryotes
Primitive cells with a simple structure and no membrane-bound organelles.
Eukaryotes
Complex cells with membrane-bound internal structures.
Mitochondria
The process of energy production in the form of ATP; glucose + oxygen → CO2 + Water + energy.
Chloroplast
Organelle in plants where photosynthesis takes place.
Cell Membranes
Controls the passage of water and other molecules into or out of the cell.
Lipid Component
Fluid component of the cell membrane made of two layers of phospholipids.
Protein Component
Molecules scattered throughout the lipid bilayer forming channels.
Carbohydrates
Attached to the cell membrane and play a role in recognition.
Hydrophilic Head
The 'head' of the phospholipid.
Hydrophobic Tail
The tail of the phospholipid.
Cell membrane
The sack/border of the cell
Cytoplasm
Fluid within the cell
Organelles
The floating things each serving a different purpose
Nucleus
Contains the cells genetic information needed for growth, repair, and responsible for telling the cell what to do.
Nucleolus
Makes Ribosomes
Ribosomes
Made of ribosomal RNA and protein, make proteins, found attached to the endoplasmic reticulum
Endoplasmic Reticulum (ER)
Network of flattened, interconnected membranes connected to nucleus
Rough ER
Has ribosomes; Makes and modifies protein
Smooth ER
No ribosomes; Make lipids (Fat)
Golgi Body
Made of flat membrane sacs stacked on top of each other. Job is to process and package substances the cell has made
Lysosomes
Digest cell waste. Membrane bound sacs that contain digestive enzymes. Needed to break down other substances
Simple Diffusion
Molecules move from area of HIGH to LOW concentration
Diffusion
PASSIVE process which means no energy is used to make the molecules to move
Diffusion through a membrane
Solute moves DOWN the concentration gradient (HIGH to LOW)
Facilitated Diffusion
Molecules move through a cell membrane through a channel protein
Channel proteins
embedded in the cell membrane and have a pore for materials to cross
Carrier proteins
can change shape to move material from one side o f the membrane to the other
Osmosis
Diffusion of water across a membrane
Isotonic
A solution whose solute concentration is the same as the solute concentration inside the cell
Hypotonic (LOW)
A solution whose solute concentration is LOWER than the solute concentration inside a cell
Hypertonic (HIGH)
A solution whose solute concentration is HIGHER than the solute concentration inside a cell
Concentration Gradient
The concentration gradient is the difference in concentration of a substance across a membrane
Surface Area
Surface Area will determine the exchange of materials between the cell and its environment
Active Transport
Active transport is the movement of molecules form a region of LOW concentration to a region of HIGH concentration
Phagocytosis
Solid particle
Pinocytosis
Fluid
Endocytosis
WHEN A SOLID/FLUID IS GOING INTO THE CELL
Exocytosis
WHEN A SOLID/FLUID IS GOING OUT OF THE CELL
Magnification
The degree to which an image is enlarged compared to the original object.
Resolution
The ability to distinguish between two points that are close together; clarity of an image.
Condenser Lens
Concentrates light onto the specimen in a microscope.
Objective Lens
The primary lens that magnifies the specimen in a microscope.
Ocular Lens
The eyepiece lens that further magnifies the image from the objective lens.
Artefacts in Microscopy
False structures or features introduced during sample preparation that are not present in the original specimen.
Compound Light Microscope
200 nm,
15,000x resolution
Fluorescence Microscope
Modified light microscope that highlights specific parts of a cell. High-intensity light source causes fluorescent parts of cell to light up. Useful for seeing structures beyond the resolution limit of standard light microscopes.
Electron Microscopes
Use electron beams.
much higher magnification
greater resolution
Can view subcellular
Scanning Electron Microscope (SEM)
Electrons bounce off the surface of a specimen.
Produces 3D images of surfaces.
Resolution: 10 nm
Confocal Laser Scanning Microscope
Uses a laser to scan the specimen point by point at different depths. Creates detailed 3D images of intact cells. Useful for studying the structure of cells without damaging them.
Limitations of Electron Microscopes
Cannot view living specimen
Expensive and large; require high maintenance.
Light microscope resolution range
Can’t see below 200 nm. Can’t show organelles in depth.
Chemicals In Cells
Chemicals and ions required for cells to function, split into organic and inorganic groups.
Organic Chemicals
Contain Hydrogen, Oxygen, Carbon; derived from living organisms. Includes carbohydrates, lipids, proteins, and nucleic acids.
Monosaccharides
Basic subunit of carbohydrates.
Disaccharides
Two monosaccharides joined together.
Polysaccharides
More than two monosaccharides joined together.
Lipids
Energy storage molecules, including fats and oils.
Proteins
Contain nitrogen, carbon, oxygen, and hydrogen; more complex than lipids and carbohydrates. Subunits are amino acids joined by polypeptide bonds.
Nucleic Acids
Genetic material of all organisms; includes DNA and RNA. Subunits are nucleotides.
Inorganic Chemicals
Includes water, oxygen, carbon dioxide, and nitrogen.
DNA
Genetic material of all organisms
RNA
Genetic material of all organisms
Hetertrophs
‘consumers’, eat organic matter
Autotrophs
Make their own food, plants
what are Enzymes
Catalysts of chemical reactions,
Protein
Each enzyme works on SPECIFIC substrate
What is the active site
Region where reaction occurs, substrate binds with enzymes
Induced fit model
Enzyme molds shape to fit the substrate
Lock and key model
Enzyme doesn’t change shape, substrate is tight
Enzyme vs temperature
Optimal = 37*C
Denatures at 40*C
Enzyme vs pH
optimal = 7
above 7, enzyme becomes less efficient
Enzyme vs substrate concentration
½ Vmax + Vmax
All substrates sites are active = plateu
Purpose of Xylem
Transport water from roots to rest of plant
Purpose of phloem
Transport sugars and nutrients around plant from sources to sinks
Sinks
Anywhere sucrose is used or removed
Sinks
Anywhere sucrose is produced (chloroplast)
what is the Cohesion tension model
model explaining how water can travel up plant
what is cohesion
tendency for water molecules to stick together
what is adhesion
tendency for water molecules to stick to other substsances
What is active transport
Survival mechanism
Low to high
Protein channel
Passive transport
No energy required,
High to low
facilitated diffusion
passive but molecules are too big, must go through channel protein
Stomata
underside of leaf
Diffusion of gasses occurs
surrounded by guard cells