MICROBIO LAB FINALS

Triple Sugar Iron (TSI) test

tubed differential medium for detecting carbohydrate fermentation, gas, and hydrogen sulfide (H2S) production

Enterobacteriaceae

family that are typically identified using TSI

aerobic

the slant of TSI tube is?

anaerobic

the butt of TSI tube is?

• glucose (0.1%)

• sucrose (1%)

• lactose (1%)

sugars in TSI

yellow

what color does TSI turn into when there is acid?

phenol red

what color does TSI turn into when sugars don’t ferment/alkaline?

black

what color does TSI turn into when there is hydrogen sulfide production?

splitting

what happens in TSI tube when there is presence of gas?

slant/butt, H₂S (?), G (?) or NC/NC

how to name TSI agar slant

NC/NC

name this TSI tube

A/A, H₂S, G

name this TSI tube

A/A. H₂S

name this TSI tube

K/A, H₂S, G

name this TSI tube

K/A, H₂S

name this TSI tube

K/A, G

name this TSI tube

K/A

name this TSI tubeA/

A/A, G

name this TSI slant

A/A

name this TSI tube

K/K

name this TSI tube

non-fermenter

what does NC/NC result in TSI mean?

glucose fermenter

what does K/A (alkaline over acid) result mean in TSI?

• Yersinia (Y. pseudotuberculosis, Y. pestis)

• Shigella spp.

• Salmonella spp. (w/ H₂S production)

glucose fermenters in TSI

glucose, lactose and/or sucrose fermenter

what does A/A (acid over acid) result in TSI mean?

• Yersinia enterocolitica

• E.coli (w/ gas)

• Klebsiella (w/ gas)

• Proteus vulgaris (w/ H₂S production)

glucose, lactose, and/or sucrose fermenters in TSI slants

glucose, lactose, and sucrose nonfermenter

what does K/K result mean in TSI slant?

Pseudomonas spp.

glucose, lactose, and sucrose nonfermenter in TSI

S: -ve

I: +ve

M: +ve

MRT: +ve

VPT: -ve

CUT: -ve

Escherichia IMViC test results

S: -ve

I: -ve

M: -ve

MRT: -ve

VPT: +ve

CUT: +ve

Klebsiella IMViC test results

S: +ve

I: -ve

M: +ve

MRT: +ve

VPT: -ve

CUT: +ve

Salmonella IMViC test results

S: -ve

I: +ve

M: +ve

MRT: +ve

VPT: -ve

CUT: -ve

Shigella IMViC test results

S: +ve

I: +ve

M: +ve

MRT: +ve

VPT: V

CUT: V

Proteus IMViC test results

S: -ve

I: V

M: +ve

MRT: +ve

VPT: -ve

CUT: -ve

Yersinia IMViC test results

indole test

detects production of indole produced from hydrolysis of tryptophan by bacteria

tryptophan

essential amino acid metabolized by bacteria

kovac’s reagent

reagent used in indole test

negative

what is the result of this tube in indole test?

positive

what is the result of this tube in indole test?

methyl red test

performed in conjunction with voges-proskauer test; examines carbohydrate metabolism of bacteria

mixed acid pathway

methyl red test detect what?

negative

what result is this in methyl red test?

positive

what is this result in methyl red test?