IMMUNOHISTOCHEMISTRY PART 2- LN

KERATIN

Highly sensitive marker for epithelial cells (Epithelium tumors)

Cytokeratin 7 (CK7)

Carcinomas of the lung, breast, uterus and ovaries (serous tumors)

Cytokeratin 20 (CK20)

Carcinomas of colon and stomach

CK7 and CK20

Transitional carcinomas of the bladder and mucinous ovarian tumor

EPITHELIAL MEMBRANE ANTIGEN (EMA)

Determining site tumor

EPITHELIAL MEMBRANE ANTIGEN (EMA)

Breast, lung and kidney adenocarcinomas

CARCINOEMBRYONIC MEMBRANE ANTIGEN (EMA)

Differentiating adenocarcinoma and mesothelioma

CARCINOEMBRYONIC MEMBRANE ANTIGEN (EMA)

GIT, pancreas, lung, breast, ovary, uterus, and cervix

CARCINOEMBRYONIC MEMBRANE ANTIGEN (EMA)

Thyroid, lung and neuroendocrine tumors

THYROID TRANSCRIPTION FACTORS (TTF-1)

Distinguishing lung adenocarcinoma from mesotheliomas

PROSTATE SPECIFIC ANTIGEN (PSA)

Prostatic carcinoma, pancreatic and salivary gland tumor

ACTIN

Muscle differentiation (smooth, skeletal and cardiac muscle)

VIMENTIN

Melanoma and Schwannomas

DESMIN

Myogenic tumors (leiomyoma and rhabdomyosarcoma)

GLIAL FIBRILLARY ACIDIC PROTEIN (GFAP)

Astrocytoma

NEUROFILAMENT (NF)

Neuronal or neuroendocrine differentiation

S100 PROTEIN

Calcium-binding protein that is expressed in CNS goal cells, Schwann cells, melanocytes, chondrocytes, skeletal and cardiac muscle and myoepithelial cells

NEURON-SPECIFIC ENOLASE (NSE)

Neural or neuroendocrine differentiation

CHROMOGRANIN

Neuroendocrine differentiation

SYNAPTOPHYSIN

Associated with presynaptic vesicles of neurons; identified in normal neurons and neuroendocrine cells.

MUSCLE-SPECIFIC ACTIN AND MYOSIN; Myo-D1, myoglobin and myogenin

Myogenic tumors (Skeletal muscle)

CD68 OR FAM 56; alpha-1-antitrypsin and alpha-1-antichymotrypsin

Fibrohistiolyitc tumors

ENDOTHELIAL MARKERS; FACTOR VII - related antigen, CD31 and Ulex Europaeus (UEA)

Vascular tumors (Angiosarcoma)

S100 PROTEIN MELANOSOME (HMB-45) MELAN-A (MART -1)

Melanomas

BEST: LEUKOCYTE COMMON ANTIGEN (LCA) OR CD45

Lymphomas