Cells & pathogens, Biomolecules, Cellular respiration & Transport, Alveoli in lungs
Magnification
how much bigger than real life an image is
worked out with a microscope by multiplying the two lenses together
Resolution
smallest distance between two points where they can still be seen as separate
Field of view
circular area in a microscope’s view
Scale bars
on the sides of images, used to estimate sizes
Magnification equation
image / actual
Electron microscope
type of microscope that uses beams of electrons as a source of illumination and is much more powerful than a light microscope
How many millimetres (mm) in a metre?
1,000
How many micrometers (μm) in a metre?
1,000,000
How many nanometers (nm) in a meter?
1,000,000,000
How many picometers (pm) in a meter?
1,000,000,000,000
Eukaryotic cell
a cell with a nucleus
Cell membrane
thin bag, controls what enters/leaves
Cytoplasm
watery jelly, where cell’s activities occur
Mitochondria
where aerobic respiration occurs
Nucleus
controls cell, contains chromosomes & DNA
Ribosome
makes new protiens
Cell wall
(plant cell only) supports the cell, made of cellulose
Chloroplast
contains chlorophyll, used for photosynthesis
Vacuole
stores cell sap that keeps cell firm and rigid
Specialised cells
cells that have a function - their sub-cellular structures have adapted to their function
Specialised cells for digestion
have membranes with tiny folds (microvilli) which increase the surface area, allowing for faster absorption
Specialised cells for reproduction (sperm cells)
streamlined shape
lots of mitochondria for energy
tail for swimming
acrosome (helps them burrow into the egg cell)
only one set of chromosomes
Specialised cells for reproduction (egg cells)
nutrients in cytoplasm
changes to cell membrane after fertilisation to stop more sperms coming in
only one set of chromosomes
Diploid cells
cells with two sets of chromosomes
Haploid cells
cells with one set of chromosomes
Flagellum
spins round to move a cell (bacteria)
What do bacteria not have that other cells do?
nucleus, chromosomes, mitochondria, chloroplasts
What do bacteria have in place of things like the nucleus?
chromosomal DNA - controls cell’s activities
Slime coat (on bacteria)
for protection (not on all bacteria)
Flexible cell wall (on bacteria)
for support (not made of cellulose)
Plasmid & chromosomal DNA (in bacteria)
controls the cell’s activities in place of the nucleus
Pathogens
disease causing organisms. they produce toxins that damage cells, or invade them and change their functions
Why aren’t viruses true organisms
they don’t have a cellular structure
How do viruses cause disease
they infect a cell and take over its DNA & processes to make new viruses
Oral route
when a pathogen enters the body through the mouth
Capsids
strands of genetic material with a protein coat
Lysis
complete breakdown of a cell
Lytic cycle definition
life cycle of a virus that causes lysis
Lysogenic cycle definition
life cycle of a virus that inserts genetic material into a cell
Lytic cycle steps
virus attaches to cell & inserts genetic material
new genetic material & proteins created and assembled
cell lyses, releasing viruses
repeat
Lysogenic cycle steps
genetic material of virus inserts into chromosome
bacteria reproduces, replicating virus material too
virus material separates from chromosome
repeat
This doesn’t cause lysis
Test for starch
Iodine, turns blue-black for positive
Test for reducing sugar
benedict’s, red for positive, orange/yellow/green for less
Test for protein
biuret, purple for positive, lighter for less
Test for lipids (aka fats)
ethanol (emulsion test), cloudy/milky for positive
Health
a state of complete physical, mental and social well being and not just the absence of disease
Symptoms
things that give you cues as to which cells/organs are infected
Treatments
things that target diseased cells to try to help them get better
Non-communicable diseases
diseases that can’t be spread from one person to another e.g. heart attacks
Communicable diseases
diseases that are caused by pathogens
Four groups of pathogens
viruses, bacteria, fungi, protists
Cell
The smallest unit of life, carry out all life processes, all organisms are made of them
Cholera
Bacteria
Causes diarrhoea
Spread through water
Prevent by washing hands, cooking food, drinking clean water
Tuberculosis
Bacteria
Causes lung damage
Spread in air
Prevented by wearing masks, having good ventalation
Chalara ash dieback
Fungus
Causes leaf loss, bark lesions
Spread in air
Prevented by destroying plants
Malaria
Virus
Causes damage to blood and liver
Spread through animal vectors
Prevented with insect repellent
HIV
Virus
Causes destroyed white blood cells, leading to weakened immune system and onset of AIDS
Spread from body fluids
Prevented by having safe sex, getting tested
Helicobacter Pylori
Virus
Causes stomach ulcers
Spread through oral transmission
Prevented by cooking food
Ebola
Virus
Causes haemorrhagic fever
Spread from body fluids
Prevented by not touching bodies and isolated
Chlamydia
Bacteria
Causes pain while peeing, unusual discharge
Spread through unprotected sex
Prevented by getting tested & treated
Monomer
building block of a substance
Monomer of carbohydrates
Sugars
Monomer of lipids (fats)
fatty acids & glycerol
Monomer of proteins
amino acids
Function of carbohydrates
energy
Function of lipids (fats)
insulation, energy, cell membranes
Function of proteins
muscles, immune systems, enzymes
Enzymes
things that speed up processes without being used up
Denature
when a protein changes shape from extreme temp/pH and can’t work
Active site
where the enzyme and substrate join together
Specificity
one type of enzyme for one type of substrate
Product
the result(s) of the reaction
Enzyme-substrate complex
structure formed when enzyme and substrate come together
Catalyst
something that speeds up a reaction without being used up (e.g. an enzyme)
Catalyses
speeds up
Substrate that amylase breaks down
starch
Enzyme that is broken down by starch
amylase
Substrate that lipase breaks down
lipids
Enzyme that is broken down by lipids
lipase
Substrate that protease breaks down
protein
Enzyme that is broken down by protein
protease
Ending of all names of enzymes
ase
Optimum
the temp/pH where the enzyme is working the best/most
How enzymes work
The substrate binds to the enzyme forming an enzyme-substrate complex
Stress is placed on the bonds in the substrate and they break
Products are released and the enzyme can bind other substances
Rate of reaction definition
relative speed at which reaction takes place
Units of rate
s-1
What goes on the y axis of a rate graph
rate
What goes on the x axis of a graph
what you’re changing e.g. temperature
Dependent variable
what you measure, on the y axis
Independent variable
what you change, on x axis
What binds with A
T
What binds with D
G
What do you need in a nucleotide
base, sugar, phospahte
Name of the shape DNA
double helix
Gene
section of a DNA molecule
Genome
Name for all genes in a cell
Where in the cell’s DNA found
in the nucleus
Extracting DNA practical
Break the cells open to get to the DNA (by mashing it)
Filter the debris e.g. the cell walls out and collect the nuclei
Add soapy water to dissolve the cell membranes and the nuclear membrane, which are made of lipids
Use ice cold ethanol to extract the DNA, you will get some slimy stuff which is the DNA
Cellular respiration
A series of chemical reactions that take place continually in all cells. Glucose is broken down releasing energy. It is an exothermic reaction so the heat produced keeps animals warm.
ATP
short term storage molecule for metabolism