Lecture 22: Eukaryotic Genes and Genomes III

Vocabulary flashcards covering key concepts from the lecture on eukaryotic genes and genomics, focusing on S. cerevisiae and gene regulation.

S. cerevisiae

A species of yeast known as baker's or brewer's yeast used in genetic studies.

Gal regulation

A system of gene regulation in S. cerevisiae that responds to the presence of galactose and the absence of glucose.

Global transcriptional profiling

A technique that allows monitoring the mRNA expression levels for every gene in S. cerevisiae in one experiment.

Northern Blot Analysis

A technique used to measure RNA levels based on the hybridization of complementary sequences.

Microarray

A technology used to analyze mRNA levels by hybridizing labeled mRNA to immobilized DNA probes on a solid surface.

Chromatin Immuno Precipitation (ChIP)

A method that identifies the binding sites of transcription factors to DNA in living cells.

Gal4

A transcriptional activator in S. cerevisiae that binds to UAS and URS sequences to regulate gene expression.

Up-regulated genes

Genes whose expression levels increase under specific conditions, such as the presence of galactose.

Interactomes

Maps of protein-protein interactions within a biological organism.

Pair-wise interactions

Tests of interactions between pairs of proteins to study their relationships within the cellular environment.