haemo and gram neg

Haemophilus spp.

-gram neg coccobacilli

-catalase: pos

-oxidase: pos

-non motile

-nitrate red: neg

-req factor X and V for growth (typically)

Hib

H. influenzae serotype

-systemic inf

-meningitis kids 3 mo-6 yrs

NTHi

H. influenzae type

-non encapsulated

-localized inf

-conjunctivitis

-leakage into CNS (meningitis in immunocomp, neonatal sepsis)

H. influenzae

-Hib and NTHi

-CHOC (X and V)

-aerobic, 5% CO2

-grey/white colonies

-zone III, IV

H. aegyptius

-pink eye

-genetic relation to influenzae

-indole and xylose: neg

biogroup aegyptius

-non encapsulated H. influenzae

-pink eye in kids

-Brazilian purpuric fever

-indole and xylose: pos

H. ducreyi

-genital ulcer disease (chancroid)

-”school of fish”

-X req

-zone I, III, IV

-GC with bovine Hgb, 5-10% CO2, high humidity

Aggregabacter aphropholis

-oral flora

-endocarditis

-alpha hemolysis

-V req

-porphyrin: pos (makes X)

-CTA: all

H. parainfluenzae

-oral flora

-progressive endocarditis: mitral valve

-zone II, III, IV

-V

-prophyrin: pos

H. parahaemolyticus

-normal flora

-pharyngitis

-zone II, III, IV and beta hemolysis

-V 

-porphyrin: pos

H. haemolyticus

-V

-zone III, IV and beta hemolysis

A. actinomycetemcomitas

-oral flora

-peridontis

-virulence: collagenase, leukotoxin

-catalase pos

-star shaped on media

Cardiobacterium hominis

-nose, mouth, throat, GI flora

-oral inf → endocarditis with no fever (biofilms)

-rosettes and long filaments on gram

-dark grey, spready

-oxidase: pos

-indole: pos

-ODC: pos

Eikenella corrodens

-oral flora

-human bites: “clenched fist wound”

-oxidase: +

-pits the agar (corrosive)

-assachrolytic

Kingella kingae

-oropharynx flora

-osteoarthritis inf in <4 yrs

-endocarditis (immunocomp)

-non motile (twitching)

-short plump rods in chains

-clear colonies on SBA

-beta hemolysis

-oxidase: pos

Kingella dendrificans

-oropharynx flora

-endocarditis

-oxidase: pos

Capnocytophaga ochracea

-oral flora

-septicemia w preexisting neutropenia

-CHOC: “gliding” and yellowish

-thin fusiform rods

-catalase: neg

-oxidase: neg

-CTA: pos (not sucrose)

Capnocytophaga canimorsus

-canine oral flora

-catalase: pos

-oxidase: pos

Pasturella multocida

-cat bites

-bipolar staining

-non motile

-facultative anaerobe

-no growth on MAC

-SBA, CHOC

-catalase: pos

-oxidase: pos

-indole: pos

Streptobacillus monoliformis

-rat bite fever

-blood, CSF, synovial, lesions, abscesses

-rash on palms and soles

-BHI with 20% horse serum

-puff ball in thioglycolate broth

-gram var, monoliform swellings

-ID with agglutination

Brucella spp.

-category B bioterrorist

-undulant fever

-aerosols, unpasteurized dairy

-aerobic (no CO2)

-catalase: pos

-oxidase: pos

-facultative intracellular pathogen

B. melitensis

-goat/sheep

-brucellosis

-neg: H2S, urease, T, F

B. abortus

-cattle

-H2S: pos

-urease: pos <2 hr

-thionine: S

B. suis

-swine

-H2S: pos

-urease: pos (<30 min)

-fuschin: S

B. canis

-dogs

-urease: pos (<30 min)

-fuschin: S

Francisella tularensis

-rabbits

-tularemia

-category A bioterrorist

-nodular lesions

-media: cysteine or thiosulfate (CHOC, TM, BCYE, thoglyc broth)

-beta lactamase: pos

-oxidase: neg

-catalase: weak pos

-urease: neg

Legionella pneumophilia

-water

-intracellular pathogen

-mild resp disease

-Leigonnaires: fever and pneumo

-Pontiac fever: fever only

-lung samples, urine, water

-media: L-cystine (CHOC w, BCYE)

Bordetella pertussis

-whooping cough

-virulence: FHA, pertactic (resp inv→ciliated epi attachment); PT (exotoxin); CyaA (cell and immune inhib); tracheal cytotox

-childhood

-calcium alginate swab

-transport media: Aimes w charc or casamino acid

-Bordet-Gengon potato inf

-Regan-Lowe (mercury droplets)

-Stainer Scholte

-low CO2