6.6 altering the function of specific genes by design p3

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7 Terms

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gene mutation

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homologous recombination

transfected disrupt constructs can inactivate specific target genes in yeast

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normal yeast gene

can be replaced with mutant alleles by homologous recombination

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PCR primer construction

generating a disruption construct containing a selectable marker gene

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how do kanMX and G418 resistance relate to yeast transformation?

the kanMX gene provides resistance to kanamycin , allow for the selection of transformants in E coli, while G418 resistance enables the selection of transformants in yeast

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diploid saccharomyces cells transformed with the disruption construct

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