To investigate the effect of heat denaturation on the rate of enzyme activity

Briefly explain the term enzyme

biological catalyst

State one advantage of immobilising enzymes

reusable

What is meant by specificity in enzymes

only acts on type of substrate

Name one factor other than temperature which affects the rate of activity of an enzyme

pH

Explain the term optimum activity in relation to enzymes

when an enzyme is working at its maximum rate

Name the enzyme, substrate and product you used to investigate the effect of heat denaturation on the rate of enzyme activity

Enzyme: Catalase

Substrate: Hydrogen Peroxide

Product: Water and Oxygen (Foam)

Describe how you carried out this investigation

• Place 10cm³ of boiled catalase in a graduated cylinder

• Add buffer pH 9 and a drop of washing-up liquid

• Add 10cm³ of hydrogen peroxide to a test tube

• Leave both in a water bath at 25deg celsius for 5 minutes

• pour hydrogen peroxide into graduated cylinder and record the volume of foam produced.