Bio Post Lab Quiz-Microscope skills

Robert Hooke (1665)

Used three lenses and a stage light to view cork, coining the term ‘cells”.

Antoine Van Leeuwenhoek (1667)

Developed a 300x single lens microscope and became known as the “father of microbiology”

Stage knob

Microscope structure used to adjust the horizontal and vertical direction of the stage.

Total Magnification Calculation

Objective Lens Power multiplied by Ocular Lens (Eyepiece) power.

Fine Focus Knob

Microscope structure used to sharply focus an image.

Storage Objective Lens

The 4X (scanning) objective lens should be in place when putting the microscope away.

Stereo Microscope

Also called a dissecting microscope; provides up to 300x magnification to view texture and fine details of larger items.

Confocal Microscope

Uses laser light and a dichromatic mirror to scan specimens create clear 3D images on a computer.

Scanning Electron Microscope (SEM)

Uses electrons on vacuum-sealed, gold-coated samples to produce black and white 3D images with high magnification.

Transmission Electron Microscope (TEM)

Uses electrons like a SEM but creates 2D images, frequently used for materials, metals, and nanotechnology.

Computational Biology

Field using biological data, computational algorithms, and models to visualize and understand structures too small for microscopes.

Carrying a Microscope

Always carry with two hands: one hand on the arm and one on the base

Preventing microscope damage

Always preventing the microscope from the side when adjusting the objective lens or stage to prevent collisions.

Microscope Lens Cleaning

Always clean objective lenses when using special microscope lens paper prior to use.