UNT Principles of Biochemistry Exam 4: Chapter 17

What are the reactions and order of Glycolysis?

1. Oxidation of glyceraldehyde

2. Transfer of a phosphate group

3. Isomerization

4. Dehydration

5. Transfer of a phosphate group

T/F: The oxidation of glyceraldehyde-3-phosphate gives a 1,3-bisphosphoglycerate

True

The transfer of a phosphate group from 1,3 -bisphosphoglycerate to ADP gives a:

3-phosphoglycerate

Which reaction from glycolysis converts a 3-phosphoglycerate to a 2-phosphoglycerate?

Isomerization

T/F: Dehydration of 2-phosphoglycerate gives pyruvate

False, it gives phosphoenolpyruvate.

What gives pyruvate?

The transfer of a phosphate group from phosphoenolpyruvate to ADP gives pyruvate

The phosphorylation of glucose to give glucose-6-phosphate (ATP is the source of the phosphate group) is known as

Hexokinase

The isomerization of dihydroxyacetone phosphate to give glyceraldehyde-3-phosphate is known as:

Triose phosphate isomerase

The transfer of a phosphate group from 1,3-bisphosphate to ADP to give 3-phosphoglycerate is known as:

Phosphoglycerate kinase (1st substrate-level phosphorylation)

Phosphorylation from ATP to fructose-6-phosphate to give fructose-1,6-bisphosphate

Phosphofructokinase

Isomerization of glucose-6-phosphate to give fructose-6-phosphate

Glucose phosphate isomerase

Cleavage of fructose-1,6-bisphosphate to give two 3-carbon fragments, glyceraldehyde-3-phosphate and dihydroxyacetone

Aldose

Oxidation (& Phosphorylation) of glyceraldehyde 3-phosphate to give 1,3-bisphosphoglycerate.

Glyceraldehyde 3-phosphate isomerase

Isomerization of 3-phosphoglycerate to give 2-phosphoglycerate

Phosphoglycermutase

Dehydration of 2-phosphoglycerate to give phosphoenolpyruvate. Generates high-energy PEP

Enolase

Transfer of a phosphate group from phosphoenolpyruvate to ADP (phosphorylation of ADP to ATP) to give pyruvate. 2nd substrate-level phosphorylation.

Pyruvate kinase

What are the two phases of glycolysis?

Preparatory phase and Payoff Phase

What happens in the preparatory phase?

5 reactions convert a molecule into two molecules of glyceraldehyde-3-phosphate, consuming ATP in the process. glucose into 2 molecules of glyceraldehyde-3-phosphate