Lab 8 - Soil Testing Day 3 - Nitrate Reduction and Zone of Inhibition

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Vocabulary flashcards covering soil testing concepts, zone of inhibition, and the nitrate reduction assay steps and interpretations.

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7 Terms

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CA-MRSA

Community-associated methicillin-resistant Staphylococcus aureus; can develop resistance through exposure to soil bacteria.

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Nitrate reduction

Microbial reduction of nitrate (NO3−) to nitrite (NO2−) or further nitrogen species.

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Durham tube

Inverted capillary tube placed in broth to trap gas and detect gas production during nitrate reduction or fermentation.

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Fermenter

Organism that ferments sugars to produce gas; its activity can affect nitrate reduction interpretation.

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Nitrite

NO2−; product of nitrate reduction that is detected by the A+B reagents.

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Nitrous acid

HNO2; acid formed when nitrite reacts with reagents, producing the red coloration.

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NH3, NO, N2O

Possible end products of nitrate reduction that may yield no color in this assay (ammonia, nitric oxide, nitrous oxide).