Clinical Chemistry 2 - Laboratory - 6 - Amylase - Complete

3.2.1.1

Amylase EC number

Hydrolase

Amylase classification

Starch, Glycogen

Amylase digestion or breakdown of?

a, 1-4 glycosidic bond

Bonds in sugars attacked by Amylase

Amylase

Smallest discovered enzyme

Nonspecific

Amylase is specific or non-specific for acute pancreatitis?

Alpha amylase

Amylase that can break down the alpha, 1-4 glycosidic bonds of amylose

Amylose, Amylopectin

Starch composition

Amylose

Long chain, unbranched chain of glucose molecules linked by an alpha, 1-4 glycosidic bond

Amylopectin

Polysaccharide with alpha, 1-6 linkages

50,000-55,000 Daltons

Amylase molecular weight

Calcium, Chloride

Amylase activators

Salivary isoamylase, Pancreatic isoamylase

Amylase isoenzymes

Starch

Amylase substrate

Acinar cells of pancreas, Salivary glands

Amylase major sources

Acute pancreatitis, Mumps, Parotitis, Ruptured ectopic pregnancies, Cholecystitis

Amylase-increased conditions

5-8 hours

Amylase increase in acute pancreatitis from onset of pain

24 hours

Amylase peak during acute pancreatitis

3-5 days

Amylase returns to normal when in acute pancreatitis?

Lipase

Enzyme used to determine acute pancreatitis

3.1.1.3

Lipase enzyme commission number

Non-hemolyzed serum

Amylase preferred specimen

Plasma in EDTA or Heparin or urine

Amylase other specimens that may be used aside from the preferred specimen

Alpha,1-4 glycosidic bonds

Bonds that alpha amylase can break down

Glucose, Maltose, Dextrin

Smaller units of amylose that are reducing sugars

Amyloclastic method

Amylase determination method that measures the disappearance of starch substrate. Amylose will break down the complex, therefore decreasing the color intensity

Iodine

This is attached to the starch substrate in the Amyloclastic method

Dark-blue

Color formed in the Amyloclastic method when iodine is attached to starch

Saccharogenic method

Amylase determination method that measures the appearance of products (reducing sugars)

Somogyi units

Saccharogenic method unit of expression

Milligrams of glucose released in 30 minutes at 37°C

What do Somogyi units mean in Saccharogenic method?

Saccharogenic method

Amylase determination classic reference method

Directly proportional

Saccharogenic method, relationship of amount of reducing sugars to amylase activity

Chromogenic method

Amylase determination method that measures the increasing color from the production of a product coupled with a chromogenic dye

Directly proportional

Chromogenic method, relationship of color intensity of soluble dye-substrate to amylase activity

Continuous monitoring method

Method used during laboratory for amylase determination

Continuous monitoring method

Amylase determination method that involves coupling of several enzyme systems to monitor amylase activity

pH 6.9

Optimal pH of Continuous monitoring method

P-type amylase

Pancreatic amylase other name

S-type amylase

Salivary amylase other name

Pancreatic amylase

Amylase derived from pancreatic tissue

Salivary amylase

Amylase derived from salivary gland tissue, fallopian tube, and lungs

Pancreatic amylase

Amylase isoenzyme that migrates slower

Salivary amylase

Amylase isoenzyme that migrates most quickly, lighter than P-type

Pancreatic amylase

Amylase isoenzyme that does the major digestive action of starches once the polysaccharides reach the intestine

Pancreatic amylase

Amylase isoenzyme that predominates in normal urine

Salivary amylase

Amylase isoenzyme that begins in the mouth and has a short duration as swallowing is only a brief process

Salivary amylase

Amylase isoenzyme with highest concentration

Salivary amylase

Amylase isoenzyme that is inactivated by the acidity of the gastric contents

Wheat germ lectin

Salivary amylase main inhibitor

Alpha-(2-chloro-4-nitrophenyl)-Beta-1,4-galactopyranoyslmaltoside

Continuous monitoring method substrate

2-Chloro-4-Nitrophenol

Continuous monitoring method product

Non-hemolytic serum

Preferred specimen

EDTA or Heparinized Plasma, Urine

Other specimens aside from the preferred specimen

7 days

Serum amylase is stable for how long if stored at 20-25°C or 4-8°C

1 year

Serum amylase is stable for how long if stored at -20°C

2 days

Urine amylase is stable for how long if stored at 20-25°C

10 days

Urine amylase is stable for how long if stored at 4-8°C

3 weeks

Urine amylase is stable for how long if stored at -20°C

Bilirubin up to 40 mg/dL, Triglycerides up to 2000 mg/dL, Hemoglobin up to 2.5 g/L

Amylase determination interferences

Up to 80 U/L

Serum amylase expected value

Up to 500 U/L

Urine amylase expected value

405 nm

Amylase determination wavelength used

40 uL

Amylase determination sample volume in uL

2000 uL

Amylase determination working reagent volume in uL

60 seconds

Amylase determination lag time

60 seconds

Amylase determination kinetic interval

4

Amylase determination amount of readings

3126

Amylase determination kinetic factor

37°C

Amylase determination reaction temperature

distilled water

Amylase determination blank with?

U/L

Amylase determination expressed in conventional unit

Increasing

Amylase determination reaction direction

10.6-1500 U/L

Amylase determination measuring range

uKat/L

Amylase determination expressed in international unit

0.017

Amylase determination conversion factor from U/L to uKat/L

1.85

Amylase determination Somogyi units to IU