K325 Exam III

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34 Terms

1
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What is the function of the spinophilin protein?
Normal synaptic function
2
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What does the GST-tag bind to?
Glutathione
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How does GST bind to spinophilin?
Through cDNA
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What are the agarose beads coated in for the protein affinity lab?
Glutathione
5
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What is the name of the bacteria used in the quorum sensing lab?
V. harveyi
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What happens if the LuxO center is active?
Regulatory sRNAs are synthesized, ultimately stopping bioluminescence
7
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When is LuxO in the active state?
When it is phosphorylated
8
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What is the chemoattractant used in the chemotaxis lab?
Diacetyl
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What does diacetyl bind to?
The ODR-10 receptor in the neurons of C. elegans
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What control compound was used in the chemotaxis lab?
Ethanol
11
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What two genes is the LacZ reporter gene linked to?
unc-54 and gpa-1
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What does LacZ encode for?
Beta-gal
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What does Beta-gal cleave?
X-gal
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What does X-gal produce?
Blue coloring
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What chemotaxis score means there is maximum attraction to the target chemical?
\+1.0
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What type of protein is spinophilin?
A scaffolding protein
17
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How was GST-spinophilin introduced to bacteria if it comes from humans?
Through plasmids
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The fusion of GST and spinophilin creates a ______________ protein.
recombinant
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What lysis buffer was used in the protein affinity lab?
BPER
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What does the BPER lysis buffer include that helps it break down portions of the cell?
Lysozymes
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What type of bonds does glutathione use to attach to the agarose beads?
Covalent
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What type of worm is C. elegans?
A roundworm
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What stage will C. elegans arrest in if there is no food or nutrients in the environment?
The L1 phase
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What are the two ‘sexes’ for C. elegans?
Male and hermaphrodite
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How can male worms be distinguished from hermaphrodites?
Hermaphrodites have a very pointy tail region
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What reagent is used to arrest the worms for viewing?
Sodium azide
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The mouth of the worm is known as the ________ region.
amphid
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The tail of the worm is known as the _________ region.
phasmid
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What type of cilia is embedded in C. elegans?
Wing-like cilia
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What three types of cilia are exposed in C. elegans?
Single, double, and brush-like cilia
31
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LuxN, LuxQ, and CqsX are all ____________ of bioluminescence.
sensors
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LuxM, LuxS, and CqsS are all _____________ of bioluminescence.
synthases (producers of auto-inducers)
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What is the reporter strain in the quorum sensing lab?
TL26
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HAI-1, AI-2, and CAI-1 are all _____________.
autoinducers