K325 Exam III

What is the function of the spinophilin protein?

What is the function of the spinophilin protein?

Normal synaptic function

What does the GST-tag bind to?

Glutathione

How does GST bind to spinophilin?

Through cDNA

What are the agarose beads coated in for the protein affinity lab?

Glutathione

What is the name of the bacteria used in the quorum sensing lab?

V. harveyi

What happens if the LuxO center is active?

Regulatory sRNAs are synthesized, ultimately stopping bioluminescence

When is LuxO in the active state?

When it is phosphorylated

What is the chemoattractant used in the chemotaxis lab?

Diacetyl

What does diacetyl bind to?

The ODR-10 receptor in the neurons of C. elegans

What control compound was used in the chemotaxis lab?

Ethanol

What two genes is the LacZ reporter gene linked to?

unc-54 and gpa-1

What does LacZ encode for?

Beta-gal

What does Beta-gal cleave?

X-gal

What does X-gal produce?

Blue coloring

What chemotaxis score means there is maximum attraction to the target chemical?

+1.0

What type of protein is spinophilin?

A scaffolding protein

How was GST-spinophilin introduced to bacteria if it comes from humans?

Through plasmids

The fusion of GST and spinophilin creates a ______________ protein.

recombinant

What lysis buffer was used in the protein affinity lab?

BPER

What does the BPER lysis buffer include that helps it break down portions of the cell?

Lysozymes

What type of bonds does glutathione use to attach to the agarose beads?

Covalent

What type of worm is C. elegans?

A roundworm

What stage will C. elegans arrest in if there is no food or nutrients in the environment?

The L1 phase

What are the two ‘sexes’ for C. elegans?

Male and hermaphrodite

How can male worms be distinguished from hermaphrodites?

Hermaphrodites have a very pointy tail region

What reagent is used to arrest the worms for viewing?

Sodium azide

The mouth of the worm is known as the ________ region.

amphid

The tail of the worm is known as the _________ region.

phasmid

What type of cilia is embedded in C. elegans?

Wing-like cilia

What three types of cilia are exposed in C. elegans?

Single, double, and brush-like cilia

LuxN, LuxQ, and CqsX are all ____________ of bioluminescence.

sensors

LuxM, LuxS, and CqsS are all _____________ of bioluminescence.

synthases (producers of auto-inducers)

What is the reporter strain in the quorum sensing lab?

TL26

HAI-1, AI-2, and CAI-1 are all _____________.

autoinducers