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Phlebotomy
When a needle is used to take blood from a vein for laboratory testing.
Post Analytical
Lab analysis and measurements in the lab of the blood after it’s been drawn and sent to the lab.
Pre analytical
Procedures before the test, identity confirmation, prepping and drawing, handling and transport
Anemia
A blood disorder, not enough hemoglobin to carry oxygen through RBC’s, can cause patients in the lab to get light headed.
Edema
Excess fluid in body tissue.
Phlebitis
Vein inflammation from blood clot
OSHA
Occupational safety and health administration
PPE
Personal protective equipment (gloves)
How to call a patient back to drawing chair.
Call their name, greet them, when they come up ask for their full name and date of birth.
How do you talk to patients?
Communication and compassion.
What are the patients rights?
They have the right to refuse treatment, access billing information, say no
What should you do if you’re leaving the computer in the lab?
Log off computer when not around
Types of disinfectants to use.
70% alcohol
What is the sterilization procedure?
70% alcohol in a circle motion, allow to dry 30-60 seconds, iodide for blood cultures back and fourth motion for 60 seconds.
Antiseptic technique
prevents the spread of infection by minimizing contamination.
When do you use antiseptic technique?
Blood cultures, venipuncture, arterial puncture.
When do you use a discard tube on a butterfly needle?
When using the butterfly needle to remove any air.
Which bottle goes first in blood culture?
Aerobic
What is the order of draw?
Aerobic
1st in blood culture, detects bacteria or other organisms that grow in presence of oxygen.
Anaerobic
2nd in blood culture test. Detects anaerobic bacteria in the bloodstream
What is blood culture stat?
Because I doctor wants to get patient on antibiotics as soon as possible so getting blood draw done asap
Contact precautions
Air, droplets, gloves, PPE, proper waste disposal, gowns/lab coats
Why is there a time limit for the tourniquet? How long should it be on?
Because it could cause harm to the limb because the blood flow is lacking, it should only be on for 1 minute or less.
Phlebotomy job types
Supervisor, blood donor, transport
Where do you dispose needles
In a non poke container
What can happen if you don’t to 2 step identifier?
Could cause wrong procedure, misdiagnosis, wrong medication
When should you use hand sanitizer and when should you wash your hands?
Hand sanitize after removing gloves, you can wash hands after as well
When should you change your gloves?
Between every patient
Needle stick accidents, what do you do?
If accidentally a needle stick follow policy and procedure at facility and have your form with you. Send form to Dorinda and let her know as well. Immediately wash the wound and enforce bleeding.
Ethical standards
Treating everyone fairly and not forcing them into something they say no to
Plasma vs serum
plasma (unclotted blood, contains fibrogen, uses anticoagulated samples), serum (clotted blood, no fibrogen, use clot tube)
Anticoagulant
Calcium binding, prevents blood from clotting
Which anticoagulant should you use in certain situations
Blood bank, black (western green sedimentation rates), orange (stat)
What color tubes have anticoagulants?
Light blue, lavender, black, green, red
What tubes do you reject if it’s not completely full
Light blue top Na citrate, anticoagulants, sometimes lavender
What is the amount of blood needed in each tube?
2 and 8.5 mL
Hemolysis
Break down of RBC’s (erythrocyte destruction), can appear pink red
Tarich
Phlebotomy training
Lipemic
Patience blood appears milky white due to high concentration of fat.
What do you do after you get blood in tube?
Shake the amount of times needed and label
What’s the cheapest way to draw blood?
Straight needles
What are the gauge sizes smallest to largest?
20G, 21-22G (standard), 23G, 25G (needle stays in longer, hemolysis can occur)
What size needle do you use on small fragile veins?
20-23G
Palpitate
Feel the vein even if you see it, use index finger bc thumb has a pulse, veins are spongy and tendons are rigid a, arteries have a pulse.
Importance of anchoring and stabilizing vein
Prevents the vein from moving so no damage or pain is caused.
What should you do before patient leaves?
Hold pressure with cotton piece and finish labeling tubes.
Fistula
Access the blood stream for dialysis or other medical treatments. Permanent artery vein connection.
Mastectomy
Try to avoid, should be done on opposite arm or below iv. Ask doctor or do finger prick.
Where do you draw if a patient has a iv in their arm?
Opposite arm or below the iv don’t stick the same vein.
septicemia
Blood poisoning
What to do if tube needs chilled right after draw?
Ice slurry, water and ice not just ice.
Chain of custody, when do you use it?
Documented paper that collects what time patient came in and what time blood was collected and the date. Drug testing, forensic toxicology, and clinical trials.
Cold of glutin
Will start a cooling process after leaving body the blood will agglutinate and clot. We want to keep blood warm if tube doesn’t specify being refrigerated.
Post analytic
After test is gone through the lab, reporting of results, entering the data in the system.