Lab safety and BBP

MLT 114 Ch 2

Nosocomial

Hospital-acquired infections

Which plans are mandated by OSHA?

Chemical hygeine, hazards communication, exposure control

Inventory of chemicals, available SDS, waste disposal, and labeling requirements are among other standards outlined in which OSHA mandated plan?

Chemical Hygiene plan

Handling and preventing BBP exposure and handling of bio-hazardous waste is outlined with which OSHA mandated plan?

Exposure Control plan

Define a blood borne pathogen

An infectious microorganism found in blood and body fluids

Define a biohazard

An object or biological substance contaminated or potentially contaminated by a pathogen.

Define biosafety

Reducing the risk of accidential exposure to a biohazardous substance

Which route of transmission do BBP spread?

Paranteral

What is regulated waste?

Waste that contains biohazardous materials

What waste should be disposed on in biohazard containers?

Regulated waste that can drip or ooze if compressed, liquid biohazardous material, and dried material that can flake off.

You have just finished pipetting a sample of blood to make a smear on a glass microscope slide. The smear was so poorly done you have to dispose of it. Where are you disposing of your items?

Glass microscope slide in sharps container. Pipet tip goes in biohazard bin. Your gloves go in the regular trash.

What is a select agent?

A bioterrorism agent that poses severe health risk and harm to people.

Describe LAI and how they get transmitted

Laboratory-acquired infections are due to exposure of pathogens in a lab setting from mishandling biological materials. They are transmitted from inhalation, percutaneous, contact, or ingestion.

What are the biosafety levels from most restricted to least?

BSL-4, BSL-3, BSL-2, BSL-1

A lab that has handwashing sinks, PPE provided, and an open bench layout is at what biosafety level?

BSL-1

A lab that uses BSC, had eyewashing sinks, and self-closing lab doors while working on moderately hazardous materials is at what biosafeety level?

BSL-2

A lab that works with infectious materials that pose high risk of transmission, hands-free sinks, PPE may include respirators, and two sets of self-closing and locking doors is at what biosafety level?

BSL-3

A lab that works with highly infectious materials that are airborne transmissible and may require the use of full body positive-pressure suits is of what biosafety level?

BSL-4

How can someone be potentially exposed by a BBP?

Not wearing PPE, accidental splashing of infectious material, exposed non-intact skin, percutaneous exposure

A federally regulated code with the purpose of preventing BBP transmission

OSHA Standard 29 CFR 1910.1030

What is included in the OSHA Standard?

Exposure control plan, universal precautions, PPE, labeling, Work practices and engineering controls, HBV vaccines, training, post-exposure medical follow up, containment of regulated waste

An approach to infection control to treat all human blood and body fluids as if they were infected by BBP

Universal Precautions

Precautions to avoid transmission of infectious diseases between patients and personnel

Standard precautions

What are Universal Precautions?

Precautions to protect workers from BBP under guidance by OSHA Standards