Recombinant DNA Technology Flashcards

Flashcards covering key concepts in Recombinant DNA Technology.

What is Recombinant DNA?

DNA prepared in the laboratory by combining fragments from multiple sources, often different species.

What are restriction enzymes?

Enzymes isolated from bacteria that restrict virus replication by cleaving viral DNA.

What is restriction endonuclease?

A type of restriction enzyme that cleaves double-stranded DNA within the interior of the strands.

What is a target sequence in the context of restriction enzymes?

A short nucleotide sequence recognized by restriction enzymes within the DNA, where the enzyme cuts.

What is a restriction site?

The specific point within the target sequence where a restriction enzyme cuts the DNA strand.

What is the relationship between different endonucleases and target sequences?

Different endonucleases recognize different target sequences.