Exercise 1 - Brightfield Microscopy

What should be in place prior to viewing any slide?

The 4x objective (the red ring)

Where should the stage be prior to viewing any slide?

The stage should be all the way down

Where should the diaphragm be prior to viewing any slide?

In the 4x/10x position

What do you use to center the specimen directly above the condenser?

The stage adjustment knob

How should the coarse adjustment knob be turned?

Away from you or counterclockwise

What is the coarse adjustment used for?

To raise the stage and focus the image on the slide

What color is the 10x ring?

The yellow ring

What is the fine adjustment knob for?

To be moved slightly to focus the image properly

Once the image is focused under the 10x ring, what objective is next?

The 40x ring (the blue ring)

When do you turn the diaphragm from 4x/10x to 40x?

When the objective is on 40x

Do you use the fine or coarse adjustment knob on 40x?

Only use the fine adjustment knob to focus the image VERY SLOWLY and DO NOT touch the coarse adjustment knob

What is true about the image under 40x?

The image can sometimes be blurry

After 40x, turn the nosepiece to the side so the light is ______ the 40x and 100x objective.

Between

What should the diaphragm be on between the 40x and 100x objective?

100x light position

Where do you drop the oil on the slide?

Where you see the light shining through the slide then turn the nosepiece to the 100x objective so that it drags through the oil

What is the clean-up process for a microscope?

Turn the nosepiece to 4x, move the stage all the down, coarse adjustment knob turned towards you or counterclockwise, the diaphragm should be on 4x/10x, remove the slide, use lens paper and cleaner to clean to stage and lens

10x (ocular lens) x 4x (scanning) = ______

40x

10x (ocular lens) x 10x (low-power) = ______

100x

10x (ocular lens) x 40x (high-dry) = ______

400x

10x (ocular lens) x 100x (oil-immersion) = ______

1000x

What is a microscope that maintains the focus of a specimen even when the magnification is increased?

Parfocal

What do the coarse and fine knobs adjust the distance between?

The stage and the objective lens

The ____ the working distance of an objective lens; the ____ the magnification

greater; lower

What should you do before the oil immersion lens is rotated into place?

Center the object of interest and add a drop of oil to the slide?

What correctly describes the position of your hands while carrying a microscope to and from the laboratory bench?

Use both hands with one on the base and one around the arm

What advantage is offered when starting with the low-power lens to view a slide?

The larger field of view enables you to easily locate the specimen