Lab safety Ppt 1

What types of safety hazards are there in the lab?

Biological, Sharps, Chemical, Radioactive, Electrical, Fire/Explosive, and Physical

What agencies are involved in developing guidelines?

Centers for Disease Control and Prevention (CDC)

Occupational Health and Safety Administration (OSHA)

Clinical Laboratory Standards Institute (CLSI)

Monitors infections in the facility (tracking the infection)

Infection control

Primary objective of biological safety is preventing the completion of the “____ __ _________”

Chain of infection

What are the Standard Precautions (SP)?

Hand hygiene, Gloves, mouth, nose, and eye protection, and gowns

Patient care-equipment, environmental control, and linen

What are examples of occupational health and blood-borne pathogens?

HIV, Hep B, Hep C

Exposure through blood, other infectious materials

Needlesticks, contaminated sharps, contact with broken skin

What does PEP stand for and what does exposure mean?

Post-Exposure Prohylaxis

Exposed to blood or bodily fluids

Gloves are a substitute for hand hygiene

False

Fluid resistant lab coats must be worn completely buttoned, gloves pulled over wrist cuffs

True

When working with specimens wear a lab coat, when leaving ALWAYS remove the lab coat

True

Never centrifuge uncapped specimens

True

Closed-toed shoes that cover the entire foot are optional

False

What is the primary method of infection transmission?

Hand Contact

Urine can be poured down the sink with no splashes, afterwards sink must be flushed with water

True

When sanitizing use a daily _:_ sodium hypochlorite (household bleach) flush. It must be protected from light or else it will degrade (used on countertops)

1:10

Decontamination is used for

Contaminated non-disposable equipment

Blood spills

Areas that process blood and bodily fluids

Wiping a mop or spill is okay

False, instead, use absorbent powder or paper towels and disinfect the area with bleach or phenol solution

What objects are Sharp hazards?

Needles, Lancets, Broken glassware

A sharps container is:

Puncture-resistant

Leak proof

Conveniently located in work area

In the event of Chemical Contact:

1.Flush 15 minutes with water ONLY

2.Seek medical attention

3.Know location of eyewash and showers

4.Remove contaminated clothing immediately

Always add acid to water, never water to acid

True

Labs must have __ available

Safety Data Sheet (SDS)

Chemicals must be disposed by not following SDS directions

False, chemicals MUST be disposed by follwing SDS directions

Sinks and drains must be flushed with large amounts of water

True

Always observe for frayed cords and report overloads

True

What type of plug are the equipment’s grounded with?

Three-prong, it provides a safe path for excess electricity

What do we do in the event of electric shock?

Do not touch the person being shocked

Remove/turn off electrical source

Turn off circuit beaker

Unplug equipment

Move person away from the source using wood or glass

____ ____ must be posted in hospitals as part of compliance with several safety and accreditation standards

Evacuation routes

What does the R in RACE stand for?

Rescue: anyone in danger

What does the A mean in RACE?

Alarm: activate

What does the C mean in RACE?

Close affected area doors

What does the E stand for in RACE?

Extinguish/Evacuate: if possibly, or exit

What does the P in PASS stand for?

Pull the pin

What does the A stand for in PASS?

Aim at the base of fire

What does the first S in PASS stand for?

Squeeze handles

What does the final S stand for in PASS?

Sweep nozzle side to side

During clinical chemistry lab, a tech spills a small

amount of a corrosive reagent on the counter. While cleaning

it up, she is not wearing gloves or a lab coat. Another tech

enters and comments on the strong odor, but no chemical

spill is reported. Later, the tech who cleaned it up reports

irritation on their hand and forearm

1.What safety violations occurred in this scenario?

2.What type of PPE should be worn when handling chemical reagents?

3.Why is it critical to report chemical spills immediately, even when small ones?

4.What procedures should be followed when a spill occurs?

Gloves and lab coat were not worn, spill incident was not reported to supervisor

Gloves, lab coat, possible eye or face protection

To ensure the lab’s protocol is followed for spills, avoid further harm and contamination, follow first aid guide on SDS, provide follow up care for exposure

Follow lab safety policy and SDS instructions, may need to call housekeeping, may use a chemical spill kit, etc