Lab 13 DNA Extraction Experiment

These flashcards cover key solutions and processes involved in the DNA extraction and fingerprinting experiment.

Extraction solution (detergent)

A solution that causes cell lysis by disrupting the cell membrane, allowing cell contents to leak out.

Protease (meat tenderizer) solution

A solution that degrades proteins and DNA damaging enzymes, aiding in the separation of proteins from nucleic acids.

Cold alcohol

Causes DNA molecules to precipitate (clump together) during the extraction process.

DNA fingerprint

A unique pattern of a person's DNA that can be used for identification.

Polymorphic regions of DNA

Stretches of DNA that are highly variable between individuals, used in DNA fingerprinting.

PCR (polymerase chain reaction)

A technique used to amplify polymorphic regions of DNA.

Restriction enzymes

Enzymes that recognize specific DNA sequences and cut the DNA at those locations to produce fragments.

Gel electrophoresis

A procedure for separating DNA fragments by size, where smaller molecules move faster through a gel than larger ones.