HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

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6 Terms

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Separation Process

The interaction of different sample components with the stationary phase causing components to elute at different times, resulting in distinct peaks in a chromatogram.

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Sample Injection

The initial step where a small amount of the sample mixture is injected into a column containing stationary phase material.

3
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Mobile Phase

A liquid solvent that carries the sample through the column, which can be a mixture of solvents with varying proportions to optimize separation.

4
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Stationary Phase

The material packed in the column that interacts differently with various components of the sample, leading to separation based on polarity, size, and chemical affinity.

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Detection

The process of identifying and measuring the components as they exit the column, commonly using UV-visible spectrophotometers, fluorescence detectors, and mass spectrometers.

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Data Analysis

Examination of the chromatogram to identify and quantify the different substances present in the sample.