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yellow top
venipuncture color for:
SPS for blood culture specimens
red OR gold/tiger top (red-grey)
venipuncture color for:
serum chemistry studies
blood donor screening
infectious disease testing
green top
venipuncture color for:
plasma determinations in chemistry
lavender top
venipuncture color for:
whole blood hematology determinations
routine immunohematology testing
blood donor screening
light blue top
venipuncture color for:
coagulation studies
invert 3-4 times
number of inversions for light blue venipuncture
invert 5 times
number of inversions for red venipuncture
invert 8-10 times
number of inversions for green, lavender, and gray venipunctures
5/8”, 22-25G
vastus lateralis muscle of anterolateral thigh
IM injection for neonate (<28 days)
1”, 22-25G
vastus lateralis muscle of anterolateral thigh
IM injection for infants (1-12 months)
1”-1.25”, 22-25G
vastus lateralis muscle of anterolateral thigh
preferred IM injection for toddlers (1-2 years), also acceptable for children ages 3-10
5/8”-1”, 22-25G
deltoid muscle of arm
preferred IM injection for children ages 3-18, also acceptable for toddlers (1-2 years)
1”, 22-25G (but 5/8” can also be used)
deltoid muscle of arm
IM injection for adults (19+) weighing <130 lbs
1”, 22-25G
deltoid muscle of arm
IM injection for adults (19+) weighing 130-152 lbs
1”-1.5”, 22-25G
deltoid muscle of arm
IM injection for adult (19+) men weighing 153-260 lbs, women weighing 153-200 lbs
1.5”, 22-25G
deltoid muscle of arm
IM injection for adult (19+) men weighing >260 lbs, women weighing >200 lbs
yellow test tube stopper
additive: sodium polyanetholsulfonate
test type: blood cultures
light blue test tube stopper
additive: sodium citrate
test type: coagulation studies
red test tube stopper
additive: none
test types:
blood chemistries
HIV/AIDS antibody
viral studies
serologic tests
gold/red-gray test tube topper
additives:
clot activator
silicone serum separator
test types:
tests requiring blood serum
routine blood donor screening
infectious disease testing
green test tube topper
additive: heparin
test types:
electrolyte studies
arterial blood gases
lavender test tube topper
additive: ethylenediaminetetraacetic acid (EDTA) (anticoagulant)
test types:
hematology
blood chemistries
gray test tube topper
additive: potassium oxalate OR sodium fluoride (anticoagulant)
test type: blood glucose
Do you have latex allergies?
If the patient answers yes, wear nonlatex gloves
Have you ever had a reaction to an adhesive bandage?
If the patient answers yes, apply a piece of cotton or gauze and secure it with paper tape.
Have you ever had a mastectomy?
If the patient answers yes, perform phlebotomy on the opposite side. If it was performed on both sides, ask the provider how to proceed.
Are you taking any blood thinners or aspirin therapy?
If the patient answers yes, be careful about moving the needle around and apply pressure for at least 2-5 minutes following the blood draw. Then, apply a pressure bandage.
Do you have a port or shunt?
If the patient answers yes, draw the sample from the opposite arm.
Do you have a history of fainting associated with having your blood drawn?
If the patient answers yes, position them in a supine or semi-Fowler’s position
Hepatitis B (HepB)
Diphtheria, tetanus, and acellular pertussis (DTaP)
Diphtheria and tetanus (DT)
Haemophilus influenzae type b (Hib)
Pneumococcal conjugate (PCV13)
Influenza (IIV)
Hepatitis A (HepA)
Human papillomavirus (9vHPV)
Meningococcal conjugate (MenACWY)
Tetanus, diphtheria, and acellular pertussis (Tdap)
Tetanus and diphtheria (Td)
Meningococcal B (MenB)
Polio (IPV)
Pneumococcal polysaccharide (PPSV23)
Zoster/Shingrix (RZV)
vaccines that should be administered intramuscularly
Measles, Mumps, Rubella (MMR)
Varicella (VAR)
Polio (IPV)
Pneumococcal polysaccharide (PPSV23)
vaccines that should be administered subcutaneously
slightly rotate the bevel
proper procedure if the bevel is against the wall of the vein
slowly advance the needle until blood begins to flow
proper procedure if the bevel is partially inserted
withdraw the needle slightly
proper procedure if the needle is inserted too deep
remove the tube from the needle , withdraw and redirect the needle into the vein, and use a new tube
proper procedure if the needle is inserted beside the vein
use a smaller tourniquet and/or tube volume
proper procedure if there is a collapsed vein
try another tube that has NOT expired or been dropped
proper procedure if there is an insufficient tube volume
25-27G
1/2”-5/8”
5-15 degrees
proper needle used for ID injection
25-27G
3/8”-5/8”
45 to 90 degrees
proper needle used for SQ injection
25-31G
5/16”-1/2”
45-90 degrees
proper needle used for SQ insulin injection
18-25G
5/8”-1 1/2”
90 degrees
proper needle used for IM injection
Gently reposition the needle. If pain persists, remove the needle
14G needle
color: olive
outer diameter: 0.072”
15G needle
color: amber
outer diameter: 0.065”
16G needle
color: gray
outer diameter: 0.064”
18G needle
color: green
outer diameter: 0.050”
20G needle
color: pink
outer diameter: 0.036”
21G needle
color: purple
outer diameter: 0.033”
22G needle
color: blue
outer diameter: 0.027”
23G needle
color: orange
outer diameter: 0.025”
25G needle
color: red
outer diameter: 0.020”
27G needle
color: white
outer diameter: 0.016”