light independent reaction (calvin cycle)

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7 Terms

1
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describe the stages of the light independent reaction (Calvin cycle):

  • CO2 reacts w/ RuBP to form unstable 6C compound - catalysed by rubisco

  • 6C compound splits into 2x GP molecules

  • ATP and NADPH from LDR are used to reduce GP to triose phosphate

  • some triose phosphate used to regenerate RuBP in the Calvin cycle, using ATP

  • some triose phosphate converted to useful organic substances

<ul><li><p>CO<sub>2</sub> reacts w/ RuBP to form unstable 6C compound - catalysed by rubisco</p></li><li><p>6C compound splits into 2x GP molecules</p></li><li><p>ATP and NADPH from LDR are used to reduce GP to triose phosphate</p></li><li><p>some triose phosphate used to regenerate RuBP in the Calvin cycle, using ATP</p></li><li><p>some triose phosphate converted to useful organic substances</p></li></ul><p></p>
2
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which enzyme catalyses ADP + Pi → ATP?

ATP synthase

3
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which enzyme catalyses ATP → ADP + Pi?

ATP hydrolase

4
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discus the efficiency of the Calvin cycle:

low:

  • one hexose sugar (e.g. glucose) is made by joining 2 molecules of TP

  • 5/6 Cs used to regenerate RuBP

  • so Calvin cycle needs to happen 6x to make 1 hexose sugar

5
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<p>how does lack of light cause the amount of radioactively labelled GP to increase? what caused the amount of radioactively labelled glucose to decrease after the light was switched off?</p>

how does lack of light cause the amount of radioactively labelled GP to increase? what caused the amount of radioactively labelled glucose to decrease after the light was switched off?

  • ATP and NADPH not formed so GP is not being used to form RuBP

  • glucose used in respiration / formation of starch/cellulose

6
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<p>between 0 and 300 secs, the conc of radioactive GP is constant - why?</p>

between 0 and 300 secs, the conc of radioactive GP is constant - why?

RuBP combines w/ CO2 to produce 2x GP

7
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<p>between 300 and 380 secs, the conc of radioactive RuBP increases - why?</p>

between 300 and 380 secs, the conc of radioactive RuBP increases - why?

  • less used to combine w/ CO2 

  • less used to form GP