Oxidase, Pigment Production, Carbohydrate Slant, Catalase, Coagulase, Esculin Hydrolysis, and Hemolysis Test - 15B

What are the Question asked in the O/F test?

1. Can the organism ferment glucose?

2. Can the organism produce CO2

What are the ingredients in O/F test tubes?

Glucose, Bromothymol Blue, Vaspar, and a Durham glass tube

What is the chemical being detected in an O/F test?

Acid

How does Bromothymol Blue work?

• yellow = acidic

• green = neutral

• blue = basic

What are the questions being asked in the Oxidase Test?

1. Does the organism have Cytochrome C enzyme in the ETC?

What are he Key Ingredients in a Oxidase test?

Oxidase Reagent

What is the pH indicator in an Oxidase Test?

No indicator, uses a reagent

What is the chemical being detected in an Oxidase test?

No chemicals are being released, detecting presence of the enzyme Cytochrome C.

What are the Questions being asked in the Pigment Production test?

1. Does the organism produce a pigment?

What are the key ingredients in the Pigment Production Test

Low nutrients in Mueller-Hinton Agar

What is the chemical being detected in the Pigment Production test?

No chemical, just looking for pigment production.

What is the pH indicator for the Pigment Production test?

No pH indicator.

What are the questions being asked in the Carbohydrate Slant test?

1. Does the organism make acid aerobically from the carbohydrate?

What are the key ingredients in a Carbohydrate Slant Test?

Phenol red and a Carbohydrate.

How does the pH indicator in a Carbohydrate Slant Test work?

Phenol Red

Yellow = Acidic

Orange = Neutral

Red = Basic

What is the chemical being detected in a Carbohydrate Slant test?

Acid from aerobic metabolism (Breaking down the carbohydrate through respiration)

What is the question being asked in a Catalase Test?

Does the organism have the enzyme Catalase?

What are the key ingredients in a Catalase test?

3% H2O2

What is the chemical being detected in a Catalase Test?

No chemical, just presence of enzyme catalase seen by bubbles.

What is the pH indicator in the Catalase Test?

No pH indicator

What are the questions being asked in the Coagulase Test?

Does the organism have the enzyme Coagulase? (Coagulase clots blood plasma)

What are the key ingredients in the Coagulase Test?

Rabbit Plasma

How does the pH indicator work in a Coagulase test?

Does not have a pH indicator

What are the chemicals being detected in the Coagulase test?

None just if it has the coagulase enzyme.

What is the question being asked in the Esculin Hydrolysis test?

Can the organism hydrolyze Esuclin?

What are the key ingredients in an Eculin test?

Esculin and Iron (ferric citrate)

What are the chemicals being detected in the Esculin test?

no chemical, just the reaction with the ferric compound.

What is the pH indicator in the Esculin Test?

none

What are the question being asked in the Hemolysis test (Blood Agar)?

Can the organism breakdown RBC’s

What are the key ingredients in the Hemolysis Test?

Whole Blood

What are the chemicals being detected in the Hemolysis Test?

none

What is the pH indicator in the Hemolysis Test?

none, visual observation