MOL-BIO LAB - Pipetting Technique

TC

Holds or contains a particular volume but does not dispense that extract volume

TD

will dispense that exact volume indicated

MOHR PIPETTES

Typically made of glass and have a tapered, cylindrical shape. They have a graduated scale along their length often with fine subdivisions. At the bottom, they have a stopcock or a tap for precise control of liquid flow.

SEROLOGICAL PIPETTES

Are typically made of transparent or translucent plastic. They have a long, slender, and cylindrical shape with volume graduations marked along the length. They often come in various sizes and may have a cotton or foam plug at the top to prevent

VOLUMETRIC PIPETTE

Used to deliver a single specific volume of liquid

PASTEUR PIPETTE

Could either be a thin glass that requires a rubber bulb to draw up solutions and transfer them or a plastic disposable pipette. Maximum 1mL, doesn’t deliver exact mL

OSTWALD-FOLIN

A special pipette used in measuring viscous fluid such as whole blood

MICROPIPETTE

Used to deliver accurate volume of liquids and is calibrated regularly.

Positive displacement pipette

The piston is in direct contact with the sample, so is part of the disposable tip, not the pipette itself, to avoid cross-contamination.

Air displacement pipette

An air cushion between the piston and the sample. To aspirate liquid, the piston first descends, pushing out a volume of air corresponding to the required volume of liquid

Accuracy

Refers to how close a measurement is to the true or accepted value.

Precision

refers to how much does tje measurements of the same item are to each other

Extended Length Tips

Tips for deep receptacles

Wide Orifice

Tipfor viscous liquids

Filter tips

Tips for corrosive or toxic liquid

Low Retention Tips

Tips for viscous and low surface tension liquids

10uL Shaftguard tip

Tips for shaft and tip ejector protection on universal-tip by pipette

Capillary Tip

Disposable tip for positive displacement pipe includes inner piston

30-40C

Angle of Pipette

3x

Pre Wet the pipette tip how many times