bio lab quiz

where do you position the flame

slightly behind your work area

what’s the distance where you perform all sterile transfers

5-10 cm (2-4 inches)

when working with fire keep open tubes and plates between

you and the flame

do not touch pipette tips to

• bench

• gloves

• tube rims

how often do you change the tip of a pipette

between every transfer (no exceptions)

how do you hold tube caps

in your hands (never on the bench)

how do you keep tube openings

angled downward

when do you close tubes

Immediately after transfer

how often do you flame for sterilization

before using needles or loop and after transfer before setting aside

when do you label agar plates

before opening

what method do you use to open agar plates

clamshell method

what step does aseptic technique apply to

every step

short exposure time =

lower contamination risk

when can you open petri dishes containing bacterial or fungal growth

never unless directed by the instructor

how do you dispose of items contaminated with organisms or bodily fluids

• 10% bleach or biohazard containers

• Decontaminate slides, spectrophotometer tubes, and other glassware in 10% bleach.

• Dispose of soft items (i.e. gauze or swabs) in labeled biohazard containers.

• Dispose of all animal tissues in labeled biohazard containers.

bacteriophages

• virus that kills bacteria

• replicates inside bacteria

what is the organism that is used in SEA-PHAGES

environmental bacteria and bacteriophages that

infect bacteria

can these organisms (environmental bacteria and bacteriophages) cause disease in adults

no

how do you treat materials used for SEA-PHAGES

as if they are potentially biohazardous/aseptic techniques at all times

when can you work on SEA-PHAGES

only during scheduled laboratory sessions

and under instructor supervision.

can you remove SEA-PHAGES materials

no

what must be worn during SEA-PHAGES

personal protective equipment (PPE) including dispoable gloves

how must samples used in SEA-PHAGES be treated like

potential sources of microbial contamination

T/F disposbale gloves must be worn at all times during SEA-PHAGES

T

what do you want to avoid generating during SEA-PHAGES

aerosols during transferring, mixing, pipetting, vortexing, or centrifuging soil or enrichment samples.

soil samples must be remained

contained within designed laboratory vessels at all times.

disposable gloves should be worn when handling

• bacterial cultures

• bacteriophage lysates

• soil enrichments

• agar plates

• micropipettes or pipette tips used with biological materials

how often can you resue a pipette tip

never

how often should petri dishes containing bacterial lawns or bacteriophage plaques must be closed

at all times unless instructed

plates should be hand and viewed from

edges and viewed from outside only

all plates must be clearly labeled with

• student or group identification

• date

• experiment or protocol name

you never dispose of cultures and lysates in the

• sink

• regular trash