5. Colour reactions of amino acids

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Amino acids can be identified by specific colour reactions.

Last updated 1:59 PM on 8/31/26
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10 Terms

1
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Biuret reaction

Two peptide linkages

2
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Ninhydrin reaction

α‑Amino acids

3
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Xanthoproteic reaction

Benzene ring of aromatic amino acids (Phe, Tyr, Trp)

4
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Millions reaction

Phenolic group (Tyr)

5
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Hopkins-Cole reaction

Indole ring (Trp)

6
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Sakaguchi reaction

Guanidino group (Arg)

7
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Nitroprusside reaction

Sulfhydryl groups (Cys)

8
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Sulfur test

Sulfhydryl groups (Cys)

9
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Pauly’s test

Imidazole ring (His)

10
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Folin-Ciocalteu's test

Phenolic groups (Tyr)