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What are the three domains of life?
Bacteria, Archaea, Eukarya
what are the domains based on?
differences in DNA gene sequences
What has systematics of eukaryotes historically been based on?
mainly based on differences in morphology and physiology
why can't microbial systematics be based on the same criteria?(referring to eukarea historically systematics)
microbes have morphology, but they have very little variation in shape
what was microbial systematics based on until 1970s
biochemical testing
What are systematics of organisms based on now?
molecular sequences like DNA - tells us more
what are the strengths of this new system?(using DNA to classify)
every living thing has DNA so it is possible to compare them
DNA sequences tell us about the evolutionary history
more accurate becAuse material is abundant
allows us to classify microbes that can't be cultured
How is a eukaryotic species defined?
refers to species that reproduce sexual
Why can't this concept be used for microbial species?(refering to how to identify eukarea)
they don't produce sexually, and they can exchange genes with distantly related species
What is the eocyte hypothesis?
states that eukaryotes originated within the archaea, not a sister group to it
Who was the scientist that discovered organisms known as prokaryotes are actually two different branches of life - archaea and bacteria?
Carl Woese
What gene was used for the original phylogenic work on determining bacterial taxonomy?
16S rRNA gene
Are all archaea extremophiles?
Almost all are known as extremophiles. But most archaea are not extremophiles. Hyperthermophiles, Halophiles, Methanogens
How do bacteria and archaea differ?
archaea have different phospholipids on their membrane
Archaea have no peptidoglycan in their cell walls
Their flagella differ
What metabolic ability is only exhibited by archaea and never by bacteria?
Archaea have the ability to produce methane (methanogen)
What common chemical compound cannot be utilized by archaea for energy production?
glucose
How are archaea beneficial?
Benefit industry and technology, source of enzymes that work at extreme temperatures and pH
Methanogens are used in solid waste treatment in wastewater treatment plants