12-15, Hydrogen sulfide production, decarboxylation, phenylalanine deamination, and urease test

0.0(0)
studied byStudied by 0 people
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
Card Sorting

1/8

encourage image

There's no tags or description

Looks like no tags are added yet.

Study Analytics
Name
Mastery
Learn
Test
Matching
Spaced

No study sessions yet.

9 Terms

1
New cards

What test is SIM agar used for?

hydrogen sulfide production

2
New cards

Describe SIM agar, including

  • what it tests for

  • complex or chemically defined

  • selective, differential, or both

  • main differential component

  • Enzyme of pathway product

  • positive control

  • negative control

  • sulfide production, indole, motility

  • complex

  • differential

  • ferrous ammonium sulfate

  • cytseine desulfhydrase enzume breaks down cysteine into sulfide or create during anaerobic respiration

  • pos: black color; Proteus vulgaris

  • neg: no black color; Enterococcus faecalis but motile, Enterobacter aerogenes not motile

<ul><li><p>sulfide production, indole, motility</p></li><li><p>complex</p></li><li><p>differential</p></li><li><p>ferrous ammonium sulfate</p></li><li><p>cytseine desulfhydrase enzume breaks down cysteine into sulfide or create during anaerobic respiration</p></li><li><p>pos: black color; Proteus vulgaris</p></li><li><p>neg: no black color; Enterococcus faecalis but motile, Enterobacter aerogenes not motile</p></li></ul><p></p>
3
New cards

Describe LDC broth, including

  • what it tests for

  • complex or chemically defined

  • selective, differential, or both

  • main differential component

  • Enzyme of pathway product

  • positive control

  • negative control

  • lysine decarboxylase enzyme; breakdown of amino acids

  • complex

  • differential

  • pH indicator bromocresol purple, ph>6.8

  • lysine decarboxylase breakdown of lyseine

  • positive control: dark purple LDC, yellow DC; Enterobacter aerogenes

  • negative control: no color change anything different than purple LDC and yellow DC; Enterococcus faecalis

4
New cards
<p>Describe the test for phenylaline deamination, including<br>- what deanimation is</p><ul><li><p>what kind of substance the broth is</p></li><li><p>the process</p></li><li><p>positive results</p></li><li><p>negative results</p></li></ul><p></p>

Describe the test for phenylaline deamination, including
- what deanimation is

  • what kind of substance the broth is

  • the process

  • positive results

  • negative results

  • removal of NH3+ from a molecule by phenylalanine deaminase enzyme

  • Buffered single substance, phenylalanine tablet added

  • phenylalamine broken down by PDA enzyme into phenyl pyruvic acid and NH3

  • positive: bright green; Proteus vulgaris

  • negative: no color change Enterococcus faecalis

<ul><li><p>removal of NH3+ from a molecule by phenylalanine deaminase enzyme </p></li><li><p>Buffered single substance, phenylalanine tablet added</p></li><li><p>phenylalamine broken down by PDA enzyme into phenyl pyruvic acid and NH3</p></li><li><p>positive: bright green; Proteus vulgaris</p></li><li><p>negative: no color change Enterococcus faecalis</p></li></ul><p></p>
5
New cards
<p>Descibe the the urease test including</p><ul><li><p>the enzyme</p></li><li><p> the process</p></li><li><p>main differential indicator</p></li><li><p>positive results</p></li><li><p>negative results</p></li></ul><p></p>

Descibe the the urease test including

  • the enzyme

  • the process

  • main differential indicator

  • positive results

  • negative results

  • urease to break down urea into ammonia and CO2

  • urease breaks down urea into NH3 and CO2, increasing the pH

  • phenol red

  • pos: hot pink

  • neg: yellow (no color change)

<ul><li><p>urease to break down urea into ammonia and CO2</p></li><li><p>urease breaks down urea into NH3 and CO2, increasing the pH</p></li><li><p>phenol red</p></li><li><p>pos: hot pink</p></li><li><p>neg: yellow (no color change)</p></li></ul><p></p>
6
New cards

What are the positive and negative controls and organisms for LDC broth?

  • pos: dark purple in LDC, yellow in DC; Enterobacter aerogenes

  • neg: anything else; Enterococcus faecalis

7
New cards

What are the positive and negative controls for phenylalanine deamination?

  • pos: bright green; Proteus vulgaris

  • neg: no color change; Enterococcus faecalis

<ul><li><p>pos: bright green; Proteus vulgaris</p></li><li><p>neg: no color change; Enterococcus faecalis</p></li></ul><p></p>
8
New cards

What are the positive and negative controls for the urease test?

  • pos: pink, Proteus vulgaris

  • neg: no color change, Enterococcus faecalis

<ul><li><p>pos: pink, Proteus vulgaris</p></li><li><p>neg: no color change, Enterococcus faecalis</p></li></ul><p></p>
9
New cards

What are the positive and negative controls for the SIM agar test?

  • pos: black color; Proteus vulgaris

  • neg: no black color; Enterococcus faecalis but motile, Enterobacter aerogenes not motile

<ul><li><p>pos: black color; Proteus vulgaris</p></li><li><p>neg: no black color; Enterococcus faecalis but motile, Enterobacter aerogenes not motile</p></li></ul><p></p>